Table 2. Agonist-stimulated adenylyl... | American Society of Hematology

Table 2.

Agonist-stimulated adenylyl cyclase inhibition by the wild-type P2Y₁₂ and its mutants

	EC₅₀
	ADP, μM	2-MeSADP, nM
P2Y₁₂WT	0.32 ± 0.08	0.17 ± 0.03
P2Y₁₂Cys17Ser	0.32 ± 0.09	0.20 ± 0.03
P2Y₁₂Cys270Ser	0.26 ± 0.10	0.13 ± 0.02
P2Y₁₂Cys17Ser/Cys270Ser	0.26 ± 0.16	0.47 ± 0.16
P2Y₁₂Cys97Ser	NA^*	NA^*
P2Y₁₂Cys175Ser	NA^†	NA^†

	EC₅₀
	ADP, μM	2-MeSADP, nM
P2Y₁₂WT	0.32 ± 0.08	0.17 ± 0.03
P2Y₁₂Cys17Ser	0.32 ± 0.09	0.20 ± 0.03
P2Y₁₂Cys270Ser	0.26 ± 0.10	0.13 ± 0.02
P2Y₁₂Cys17Ser/Cys270Ser	0.26 ± 0.16	0.47 ± 0.16
P2Y₁₂Cys97Ser	NA^*	NA^*
P2Y₁₂Cys175Ser	NA^†	NA^†

The values are mean ± SE, n = 3-7.

NA indicates no activity or expression detectable among 61 clones tested.

NA indicates no activity or expression detectable among 49 clones tested.