Table 1. Comparison of total... | American Society of Hematology

Table 1.

Comparison of total hematopoietic progenitors emerging from muscle and neural tissue to changes in total number of specific phenotypically characterized subsets

	De novo	10% CEE	HGF	HGF + BMP-4 + EPO
Muscle tissue
Total cells	500 000	920 000	330 000	320 000
AC133⁺	190 000	0	11 612	28 139
CD34⁺	23 500	87 400	46 054	41 195
CD45⁺	1 025	0	0	5 165
CD34⁺CD45⁺	600	0	0	59
Total hematopoietic progenitors	8	4	182	630
Neural tissue
Total cells	500 000	120 000	110 000	82 000
AC133⁺	97 450	291	1 116	658
CD34⁺	21 650	1 087	283	789
CD45⁺	4 850	2 388	9 843	9 574
CD34⁺CD45⁺	95	669	541	132
Total hematopoietic progenitors	1	5	507	2 475

	De novo	10% CEE	HGF	HGF + BMP-4 + EPO
Muscle tissue
Total cells	500 000	920 000	330 000	320 000
AC133⁺	190 000	0	11 612	28 139
CD34⁺	23 500	87 400	46 054	41 195
CD45⁺	1 025	0	0	5 165
CD34⁺CD45⁺	600	0	0	59
Total hematopoietic progenitors	8	4	182	630
Neural tissue
Total cells	500 000	120 000	110 000	82 000
AC133⁺	97 450	291	1 116	658
CD34⁺	21 650	1 087	283	789
CD45⁺	4 850	2 388	9 843	9 574
CD34⁺CD45⁺	95	669	541	132
Total hematopoietic progenitors	1	5	507	2 475

Flow cytometry was used to assess the expression of human-specific cell surface proteins AC133, CD34, CD45, and double-positive CD34/CD45 before and after in vitro culture in serum-free media containing either 10% chicken embryonic extract (CEE) or human hematopoietic growth factors (HGFs) alone or with bone morphogenetic protein-4 (BMP-4) and erythropoietin (EPO) for a total of 500 000 human muscle– and human neural–derived cells. Values show average absolute number of cells of each phenotype. Absolute number of detected hematopoietic progenitors after culture in each medium is given. Treated human muscle and neural cells maintained normal expression of tissue-specific markers (myosin for muscle and nestin for neural tissue) irrespective of changes in hematopoietic cell surface phenotype as shown in Figure 3.

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