TCR-BV10 gene transcripts of patient CLL 402
| Population . | BV . | NDN . | BJ . | Gene segments . | Frequency . |
|---|---|---|---|---|---|
| CD8/native BV10 | C A S | G V G G | N E Q F F G | BJ1S1 | 14 of 15 |
| C A S | L E G G S | Y S N Q P Q H F G | BJ1S5 | 1 of 15 | |
| CD/8 activated BV10 | C A S | G V G G | N E Q F F G | BJ2S1 | 15 of 15 |
| Population . | BV . | NDN . | BJ . | Gene segments . | Frequency . |
|---|---|---|---|---|---|
| CD8/native BV10 | C A S | G V G G | N E Q F F G | BJ1S1 | 14 of 15 |
| C A S | L E G G S | Y S N Q P Q H F G | BJ1S5 | 1 of 15 | |
| CD/8 activated BV10 | C A S | G V G G | N E Q F F G | BJ2S1 | 15 of 15 |
cDNA from the populations indicated was PCR-amplified with BV-BC primers and cloned into vector (see “Materials and methods”). The TCR joining segments (BJ regions) were eluted by comparison with the BJ gene segments in the GenBank. The NDN-region nucleotides were defined as all nucleotides that could not have been accounted for by any of the germline BV or BJ sequences.
BV indicates TCR-β variable region segment gene.