Table 1. Analysis of the ability of Lin... | American Society of Hematology

Table 1.

Analysis of the ability of Lin⁻ cells expressing HA to reconstitute hemopoiesis in vivo

	Lin⁻ HA⁺, %^*	Lin⁻Sca⁺HA⁺, %^*
Mac-1/Gr-1	40 ± 3	68
B220	36 ± 6	UN
CD4/CD8	20 ± 3	22

Mice were injected with either 5000 to 6000 Lin⁻HA⁺ cells (4 mice) or 500 Lin⁻Sca⁺HA⁺ cells (2 mice) and analyzed 4 to 8 weeks after transplantation. Peripheral blood was collected and the red blood cells lysed using ammonium chloride. The percentage of donor cells was determined using Ly5.1 antibody labeling and was 9% ± 1.7% and 9.4% ± 0.02% following transplantation of 5000 to 6000 Lin⁻HA⁺ cells or 500 Lin⁻Sca⁺HA⁺ cells, respectively. White blood cells were labeled for macrophages and granulocytes (Mac-1/Gr-1), B cells (B220), and T cells (CD4/CD8) and the proportion of donor analyzed using an Ly5.1 antibody.

UN indicates unknown.

Percent of each subpopulation within the donor cells detected following a transplant of each subpopulation.

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