GPIIIa oligonucleotide primers
| Primer . | Sequence . |
|---|---|
| sE42K* | (195)CCTGAAGAAGAACCTGCTG(213) |
| aE42K* | (213)CAGCAGGTTCTTCTTCAGG(195) |
| sA50S* | (216)GGATAACTGTTCCCCAGAATC(236) |
| aA50S* | (236)GATTCTGGGGAACAGTTATCC(216) |
| sR62E* | (250)GTGAGCGAAGCCGAGGTACTAGAGG(274) |
| aR62E* | (274)CCTCTAGTACCTCGGCTTCGCTCAC(250) |
| sV63I* | (258)GGCCCGAATCCTAGAAGAC(276) |
| aV63I* | (276)GTCTTCTAGGATTCGGGCC(258) |
| sR62E, V63I* | (250)GTGAGTGAAGCCGAGATCCTAGAGG(274) |
| aR62E, V63I* | (274)CCTCTAGGATCTCGGCTTCACTCAC(250) |
| sD66A* | (265)GTACTAGAAGCAAGGCCCCTC(285) |
| aD66A* | (285)GAGGGGCCTTGCTTCTAGTAC(265) |
| sR67L* | (268)CTAGAAGACCTTCCCCTCAGC(288) |
| aR67L* | (288)GCTGAGGGGAAGGTCTTCTAG(268) |
| sD66A, R67L* | (268)CTAGAGGCACTTCCACT(284) |
| aD66A, R67L* | (284)AGTGGAAGTGCCTCTAG(268) |
| sD71N* | (280)CCCCTCAGTAGTAAAGGCCTCTGG(302) |
| aD71N* | (302)CCAGAGCCTTTACTACTGAGGGG(280) |
| sS50A† | (216)GGATAACTGCGCCCCAGAG(234) |
| aS50A† | (234)CTCTGGGGCGCAGTTATCC(216) |
| sE62R, I63V† | (250)GTCAGCGAGGCCCGAGTACTAGAAG(274) |
| aE62R, I63V† | (274)CTTCCTAGTACTCGGGCCTCGCTGAC(250) |
| sA66D, L67R† | (268)CTAGAAGACAGGCCGCTCAGC(288) |
| aA66D, L67R† | (288)GCTGAGCGGCCTGTCTTCTAC(268) |
| pc823 | (823)GGCTAACTAGAGAACCC(839) |
| ah540 | (540)CCCGAAGCCAATCCG(526) |
| ar540 | (540)CCCAAAGCCAATCCG(526) |
| ar221 | (221)TTATCCTTCAGCAGGTTCTTTTTC(198) |
| ar739 | (739)CCTCTGGGGCATCTCGGTTACGTG(716) |
| TOPO353 | (364)GTGTGTGCAAGCTTAGGTACCGACGGCCAGTG(353) |
| sr211 | (44)CTGCTGAAGGATAAGTGCTCCCCAGA(52) |
| Primer . | Sequence . |
|---|---|
| sE42K* | (195)CCTGAAGAAGAACCTGCTG(213) |
| aE42K* | (213)CAGCAGGTTCTTCTTCAGG(195) |
| sA50S* | (216)GGATAACTGTTCCCCAGAATC(236) |
| aA50S* | (236)GATTCTGGGGAACAGTTATCC(216) |
| sR62E* | (250)GTGAGCGAAGCCGAGGTACTAGAGG(274) |
| aR62E* | (274)CCTCTAGTACCTCGGCTTCGCTCAC(250) |
| sV63I* | (258)GGCCCGAATCCTAGAAGAC(276) |
| aV63I* | (276)GTCTTCTAGGATTCGGGCC(258) |
| sR62E, V63I* | (250)GTGAGTGAAGCCGAGATCCTAGAGG(274) |
| aR62E, V63I* | (274)CCTCTAGGATCTCGGCTTCACTCAC(250) |
| sD66A* | (265)GTACTAGAAGCAAGGCCCCTC(285) |
| aD66A* | (285)GAGGGGCCTTGCTTCTAGTAC(265) |
| sR67L* | (268)CTAGAAGACCTTCCCCTCAGC(288) |
| aR67L* | (288)GCTGAGGGGAAGGTCTTCTAG(268) |
| sD66A, R67L* | (268)CTAGAGGCACTTCCACT(284) |
| aD66A, R67L* | (284)AGTGGAAGTGCCTCTAG(268) |
| sD71N* | (280)CCCCTCAGTAGTAAAGGCCTCTGG(302) |
| aD71N* | (302)CCAGAGCCTTTACTACTGAGGGG(280) |
| sS50A† | (216)GGATAACTGCGCCCCAGAG(234) |
| aS50A† | (234)CTCTGGGGCGCAGTTATCC(216) |
| sE62R, I63V† | (250)GTCAGCGAGGCCCGAGTACTAGAAG(274) |
| aE62R, I63V† | (274)CTTCCTAGTACTCGGGCCTCGCTGAC(250) |
| sA66D, L67R† | (268)CTAGAAGACAGGCCGCTCAGC(288) |
| aA66D, L67R† | (288)GCTGAGCGGCCTGTCTTCTAC(268) |
| pc823 | (823)GGCTAACTAGAGAACCC(839) |
| ah540 | (540)CCCGAAGCCAATCCG(526) |
| ar540 | (540)CCCAAAGCCAATCCG(526) |
| ar221 | (221)TTATCCTTCAGCAGGTTCTTTTTC(198) |
| ar739 | (739)CCTCTGGGGCATCTCGGTTACGTG(716) |
| TOPO353 | (364)GTGTGTGCAAGCTTAGGTACCGACGGCCAGTG(353) |
| sr211 | (44)CTGCTGAAGGATAAGTGCTCCCCAGA(52) |
Primers used to convert human GPIIIa residues to the corresponding rat counterpart.
Primers used to convert rat GPIIIa residues to the corresponding human counterpart.
Underlined sequences represent mismatches designed to create desired mutations. In all cases “s” refers to sense, “a” refers to antisense, “h” refers to human, and “r” refers to rat. GPIIIa nucleotide numbers are shown in parentheses.