Table 5. Nucleotide sequences of VDJ... | American Society of Hematology

Table 5.

Nucleotide sequences of VDJ gene region of circulating B cell clones before and during rituximab treatment

Patients	Time-related therapy	V-region	N1	D_Hfamily	D-region	N2	J-region	J_H family
Responsive
1	Start	tgtgcaaaag	agtttgaccaggg	D4-17^*01	gagtacggtgctta	ggc	cttttgatatctgg	J3^*02
		tgtgcgagag	atggtgcgccta	D3-22^*01	aatattatgatagtaggggata	ccccattagttatt	actactttgactactgg	J4^*01
		tgtgcgaga	gcctt	D3-22^*01	tggttatgatagtagtgattattactac	t	actactgg	J4^*01
	End	tgtgcgagagg	cgggcg	D6-19^*01	gagtataggagtgcctg	ccagt	tttgactactgg	J4^*01
		tgtgcggg	cagagag	D3-3^*01	cggcttggagtggttatta	gta	accctgactactgg	J4^*03
		tgtgcgagacg	cagacagga	D4-23^*01	tgactccggtggtaa. . . . .	t	. .tggtttgactactgg	J4^*03
	1 mo follow-up	tgtgcaaaag	accgttcc	D1-26^*01	ggtataatgggagctactcc	cctcccacccccggtag	ttgacagctgg	J4^*01
		tgtgcg		D1-26^*01	gtgggggctgtga	aaagaat	gtttgactcctgg	J5^*01
		tgtgcgaaag	ataatg	D3-3^*01	agtacggtttttggagtggctataa	atggggtcgtt	actactttgactcctgg	J4^*01
		tgtgcgaaag	cccccg	D3-3^*01	aatatgagttttggagtggttat	cgggggaaatat	ttgactactgg	J4^*01
		tgtgcgagag	attttatcc	D6-19^*01	gtcagagcactggctggt	ggttaa	ttgactactgg	J4^*03
2	Start	tgtgtgaaag	atct	D3-10^*01	tattataatggttccgggagctatt	ctccctaggg	actactactactacatggacgtctgg	J6^*03
		tgtgcgaga	ggga	D6-19^*01/in	gtcccgggcagtg	gc. .	tggattactgg	J4^*01
	After first infusion	tgtgcgag	c	D6-19^*01	ttgggcagtggctggtac	gggggga	actactgg	J4^*01
	End	tgtgcgaga	ggggc	D3-3^*01	gtattacaattttttcggtg	attcttaccat	tacttggattactgg	J4^*01
	1 mo follow-up	tgtgcgaga	atc	D5-18^*01	gtggatacagctgtaatt	ttatc	tgtttttgatttctgg	J3^*02
3	Start	tgtgcgagag	aggggatacgag	D4-11^*01/in	gttacagtaacc. . . . . . . . . . . . . . . . . . . . …	ct	. . . . . .tttgactactgg	J4^*02
		tgtgtgagag.		D5-12^*01	. . . . . . .atatcggctatg. . . . . .		gccactttgactgctgg	J4^*03
		tgtgccaga	gagaggg	D6-19^*01in	. . . . . . . . . . . . . . .cccgtacgagacc	ggcccctt	actactttgactactgg	J4^*03
	End	tgtgcgagag	aggggatacgag	D4-11^*01/in	gttacagtaacc. . . . . . . . . . . . . . . . . . . . …	ct	. . . . . .tttgactactgg	J4^*01
	1 mo follow-up	tgtgcgaga	gaggggtttaa	D5-18^*01	. .ggatacagctatggttac		.cccctttgactactgg
4	Start	tgtgcgaaa	gaacagt	D4-23^*01	. .actacggtgacttc	ctcggt	. . . . . . . . . . . . . . . . . . . . .ggtatggacgtctgg	J6^*02
		tgtgcaaca	gtgccc	D3-22^*01	.tcttactatgatagtagtggttatccata		.cccctttgactactgg	J4^*03
		tgtgccaga	gagaggg	D6-19^*01	. . . . . . . . . . . . . . . .cccgtacgagacc.	ggcccctt	actactttgactactgg	J4^*02
		tgtgcgaga	ga	D2-15^*01	tcgatattgtagtgct. . . . . . . . . . . . . . . . .	ggtagctgctttg	. .actggtacttcgatctctgg	J2^*01
		tgtgcgaca	ttgtcacg	D4-17^*01	tgactacggtgactac	gtt	.tactactacaagggtatggacgtctgg	J6^*02
	End	tgtgtgaga	gatgg	D3-10^*01	gtattattatggttcggggagttacccta. .	tg	. . . . . . . . . .gacgtctgg	J4^*01
		tgtgcgaga	gac	D3-10^*01	. .atcattatgattcggggaggtattata. .		. .aactggctcgacccctgg	J5^*02
		tgtgcgaga	ggagggttagcc	D6-19^*01	gggtatagcagtggctggt. .		actactttgactactgg	J4^*01
		tgtgcgaga	gatc	D2-15^*01	. .gatattggagtggtggtagctgct. . . . . .	ttg	. .actggtacttccatctctgg	J2^*01
5	Start	tgtgcgagag	atc	D1-26^*01	ggtatagtgggag. . . . . …	a	. .tactatgactactgg	J4^*03
		tgtgcgaaag.	atag	D6-13^*01	ggacatagcagcagctgg…	cggg	. . . . . .tttgactactgg	J4^*03
		tgtgcgaaag.	a	D5-24^*01	. . . . .taggagttttgg. . . . . . . . . . . . . . . …	ccgtct	agatgcttttgatatctgg	J3^*02
		tgtgcgagag.	agggccggggcaa	D4-23^*01	tgacaaggggggtaaccc.		. . . . .ctttgattactgg	J4^*01
		tgtgcgaaa	gaagtag	D5-12^*01	gtggatatagtggctacgccccc	cca	. . . . . . . . . . . . . . . . .tacggtatggacgtctgg	J6^*02
	After first infusion	tgtgcgaga	gagggccggggcaa	D4-23^*01	tgacaagggaggta. . . . . .		acccctttgactactgg	J4^*01
		tgtgcgaga	ggagcg	D5-18^*01	ggggagacagctatgg. . . . .	aagggttt	. . . . .tttgactgctgg	J4^*03
		tgtgcgaga	gggaac	D6-13^*01	. .caccgcaactggtcc. . . . . . . . . . . . . . . . .	gaggggt	acaactggttcgacccctgg	J5^*02
		tgtgcgaaa	gaagtag	D5-12^*01	gtggatacagtggctacg. . . . . .	ccccccca	. . . . . . . . . . . . . . . . .tacggtatggacgtctgg	J6^*02
		tgtgcgag.	tc	D3-10^*01	. . . . . . . . . .aggcttcggggacttatt. . . . . .	ccggt	. . . . .tttgagttctgg	J4^*03
	End	tgtgcgaga	gagggccggggcaa	D4-23^*01	tgacaagggaggta. . . . . .		acccctttgactactgg	J4^*01
	2 mo follow-up	tgtgcgag.	ggacc	D2-15^*01	. .gattttgtagtggtggtagctgcttc…	g	. .actggtacttcgatctctgg	J2^*01
Patients	Time-related therapy	V-region	N1	D_Hfamily	D-region	N2	J-region	J_H family
	3 mo follow-up	tgtgcgag.	ggacc	D2-15^*01	. .gattttgtagtggtggtagctgcttc…	g	. .actggtacttcgattactgg	J2^*01
		tgtgcgaga	gaggaccgagactgg	D5-18^*01	agattcagcttat…		. .tattttgactactgg	J4^*03
		tgtgcgaaa	g	D5-12^*01	gtgagtatagtggttacg. . . . . .	ccccccca	. . . . . . . . . . . . . . . . .tacgggatggacgtctgg	J6^*02
			gaagtag
Unresponsive
6	Start	tgtactag.		D2-8^*02	. . . . . . . . . . .tcctggtggctt. . . . . . . . . . .	taaa	. . . . . …gactactgg	J4^*03
		tgtgcgaga	ggtaaaac	D4-17^*01	…ctacggtgactac	gcc	.ggagcttttgatatctgg	J3^*02
	End	tgtgcgaga	ggtaaaac	D4-17^*01	…ctacggtgactac	gcc	.ggagcttttgatatctgg	J3^*02
7	Start	tgtgcgaga		D6-13^*01	. .gaaacgcagcagc. . . . . . .	c	gggtgcttttgatatctgg	J3^*02
	End	tgtgcgaga		D6-13^*01	. .gaaacgcagcagc. . . . . . .	c	gggtgcttttgatatctgg	J3^*02
8	Start	tgtgcgaga	gaatatgggaca	D3-22^*01	. . . . . .gatactagtggttattactac	t	. . . . . . . . . . .actactgg	J4^*01
	End	tgtgcgaga	gaatatgggaca	D3-22^*01	. . . . . .gatactagtggttattactac	t	. . . . . . . . . . .actactgg	J4^*01

Patients	Time-related therapy	V-region	N1	D_Hfamily	D-region	N2	J-region	J_H family
Responsive
1	Start	tgtgcaaaag	agtttgaccaggg	D4-17^*01	gagtacggtgctta	ggc	cttttgatatctgg	J3^*02
		tgtgcgagag	atggtgcgccta	D3-22^*01	aatattatgatagtaggggata	ccccattagttatt	actactttgactactgg	J4^*01
		tgtgcgaga	gcctt	D3-22^*01	tggttatgatagtagtgattattactac	t	actactgg	J4^*01
	End	tgtgcgagagg	cgggcg	D6-19^*01	gagtataggagtgcctg	ccagt	tttgactactgg	J4^*01
		tgtgcggg	cagagag	D3-3^*01	cggcttggagtggttatta	gta	accctgactactgg	J4^*03
		tgtgcgagacg	cagacagga	D4-23^*01	tgactccggtggtaa. . . . .	t	. .tggtttgactactgg	J4^*03
	1 mo follow-up	tgtgcaaaag	accgttcc	D1-26^*01	ggtataatgggagctactcc	cctcccacccccggtag	ttgacagctgg	J4^*01
		tgtgcg		D1-26^*01	gtgggggctgtga	aaagaat	gtttgactcctgg	J5^*01
		tgtgcgaaag	ataatg	D3-3^*01	agtacggtttttggagtggctataa	atggggtcgtt	actactttgactcctgg	J4^*01
		tgtgcgaaag	cccccg	D3-3^*01	aatatgagttttggagtggttat	cgggggaaatat	ttgactactgg	J4^*01
		tgtgcgagag	attttatcc	D6-19^*01	gtcagagcactggctggt	ggttaa	ttgactactgg	J4^*03
2	Start	tgtgtgaaag	atct	D3-10^*01	tattataatggttccgggagctatt	ctccctaggg	actactactactacatggacgtctgg	J6^*03
		tgtgcgaga	ggga	D6-19^*01/in	gtcccgggcagtg	gc. .	tggattactgg	J4^*01
	After first infusion	tgtgcgag	c	D6-19^*01	ttgggcagtggctggtac	gggggga	actactgg	J4^*01
	End	tgtgcgaga	ggggc	D3-3^*01	gtattacaattttttcggtg	attcttaccat	tacttggattactgg	J4^*01
	1 mo follow-up	tgtgcgaga	atc	D5-18^*01	gtggatacagctgtaatt	ttatc	tgtttttgatttctgg	J3^*02
3	Start	tgtgcgagag	aggggatacgag	D4-11^*01/in	gttacagtaacc. . . . . . . . . . . . . . . . . . . . …	ct	. . . . . .tttgactactgg	J4^*02
		tgtgtgagag.		D5-12^*01	. . . . . . .atatcggctatg. . . . . .		gccactttgactgctgg	J4^*03
		tgtgccaga	gagaggg	D6-19^*01in	. . . . . . . . . . . . . . .cccgtacgagacc	ggcccctt	actactttgactactgg	J4^*03
	End	tgtgcgagag	aggggatacgag	D4-11^*01/in	gttacagtaacc. . . . . . . . . . . . . . . . . . . . …	ct	. . . . . .tttgactactgg	J4^*01
	1 mo follow-up	tgtgcgaga	gaggggtttaa	D5-18^*01	. .ggatacagctatggttac		.cccctttgactactgg
4	Start	tgtgcgaaa	gaacagt	D4-23^*01	. .actacggtgacttc	ctcggt	. . . . . . . . . . . . . . . . . . . . .ggtatggacgtctgg	J6^*02
		tgtgcaaca	gtgccc	D3-22^*01	.tcttactatgatagtagtggttatccata		.cccctttgactactgg	J4^*03
		tgtgccaga	gagaggg	D6-19^*01	. . . . . . . . . . . . . . . .cccgtacgagacc.	ggcccctt	actactttgactactgg	J4^*02
		tgtgcgaga	ga	D2-15^*01	tcgatattgtagtgct. . . . . . . . . . . . . . . . .	ggtagctgctttg	. .actggtacttcgatctctgg	J2^*01
		tgtgcgaca	ttgtcacg	D4-17^*01	tgactacggtgactac	gtt	.tactactacaagggtatggacgtctgg	J6^*02
	End	tgtgtgaga	gatgg	D3-10^*01	gtattattatggttcggggagttacccta. .	tg	. . . . . . . . . .gacgtctgg	J4^*01
		tgtgcgaga	gac	D3-10^*01	. .atcattatgattcggggaggtattata. .		. .aactggctcgacccctgg	J5^*02
		tgtgcgaga	ggagggttagcc	D6-19^*01	gggtatagcagtggctggt. .		actactttgactactgg	J4^*01
		tgtgcgaga	gatc	D2-15^*01	. .gatattggagtggtggtagctgct. . . . . .	ttg	. .actggtacttccatctctgg	J2^*01
5	Start	tgtgcgagag	atc	D1-26^*01	ggtatagtgggag. . . . . …	a	. .tactatgactactgg	J4^*03
		tgtgcgaaag.	atag	D6-13^*01	ggacatagcagcagctgg…	cggg	. . . . . .tttgactactgg	J4^*03
		tgtgcgaaag.	a	D5-24^*01	. . . . .taggagttttgg. . . . . . . . . . . . . . . …	ccgtct	agatgcttttgatatctgg	J3^*02
		tgtgcgagag.	agggccggggcaa	D4-23^*01	tgacaaggggggtaaccc.		. . . . .ctttgattactgg	J4^*01
		tgtgcgaaa	gaagtag	D5-12^*01	gtggatatagtggctacgccccc	cca	. . . . . . . . . . . . . . . . .tacggtatggacgtctgg	J6^*02
	After first infusion	tgtgcgaga	gagggccggggcaa	D4-23^*01	tgacaagggaggta. . . . . .		acccctttgactactgg	J4^*01
		tgtgcgaga	ggagcg	D5-18^*01	ggggagacagctatgg. . . . .	aagggttt	. . . . .tttgactgctgg	J4^*03
		tgtgcgaga	gggaac	D6-13^*01	. .caccgcaactggtcc. . . . . . . . . . . . . . . . .	gaggggt	acaactggttcgacccctgg	J5^*02
		tgtgcgaaa	gaagtag	D5-12^*01	gtggatacagtggctacg. . . . . .	ccccccca	. . . . . . . . . . . . . . . . .tacggtatggacgtctgg	J6^*02
		tgtgcgag.	tc	D3-10^*01	. . . . . . . . . .aggcttcggggacttatt. . . . . .	ccggt	. . . . .tttgagttctgg	J4^*03
	End	tgtgcgaga	gagggccggggcaa	D4-23^*01	tgacaagggaggta. . . . . .		acccctttgactactgg	J4^*01
	2 mo follow-up	tgtgcgag.	ggacc	D2-15^*01	. .gattttgtagtggtggtagctgcttc…	g	. .actggtacttcgatctctgg	J2^*01
Patients	Time-related therapy	V-region	N1	D_Hfamily	D-region	N2	J-region	J_H family
	3 mo follow-up	tgtgcgag.	ggacc	D2-15^*01	. .gattttgtagtggtggtagctgcttc…	g	. .actggtacttcgattactgg	J2^*01
		tgtgcgaga	gaggaccgagactgg	D5-18^*01	agattcagcttat…		. .tattttgactactgg	J4^*03
		tgtgcgaaa	g	D5-12^*01	gtgagtatagtggttacg. . . . . .	ccccccca	. . . . . . . . . . . . . . . . .tacgggatggacgtctgg	J6^*02
			gaagtag
Unresponsive
6	Start	tgtactag.		D2-8^*02	. . . . . . . . . . .tcctggtggctt. . . . . . . . . . .	taaa	. . . . . …gactactgg	J4^*03
		tgtgcgaga	ggtaaaac	D4-17^*01	…ctacggtgactac	gcc	.ggagcttttgatatctgg	J3^*02
	End	tgtgcgaga	ggtaaaac	D4-17^*01	…ctacggtgactac	gcc	.ggagcttttgatatctgg	J3^*02
7	Start	tgtgcgaga		D6-13^*01	. .gaaacgcagcagc. . . . . . .	c	gggtgcttttgatatctgg	J3^*02
	End	tgtgcgaga		D6-13^*01	. .gaaacgcagcagc. . . . . . .	c	gggtgcttttgatatctgg	J3^*02
8	Start	tgtgcgaga	gaatatgggaca	D3-22^*01	. . . . . .gatactagtggttattactac	t	. . . . . . . . . . .actactgg	J4^*01
	End	tgtgcgaga	gaatatgggaca	D3-22^*01	. . . . . .gatactagtggttattactac	t	. . . . . . . . . . .actactgg	J4^*01

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