Primers used for site-directed mutagenesis of the protein S cDNA
| Primers . | Sequence (5′ to 3′) . | Position* (5′ to 3′) . |
|---|---|---|
| France 1A | GAAACCAAACAGGATAATCTTGAAAG | 288 to 313 |
| France 1B | CTTTCAAGATTATCCTGTTTGGTTTC | 313 to 288 |
| France 2A | ATGACCCGGAAATGGATTATTTTTA | 368 to 391 |
| France 2B | TAAAAATAATCCATTTCCGGGTCAT | 391 to 368 |
| Primers . | Sequence (5′ to 3′) . | Position* (5′ to 3′) . |
|---|---|---|
| France 1A | GAAACCAAACAGGATAATCTTGAAAG | 288 to 313 |
| France 1B | CTTTCAAGATTATCCTGTTTGGTTTC | 313 to 288 |
| France 2A | ATGACCCGGAAATGGATTATTTTTA | 368 to 391 |
| France 2B | TAAAAATAATCCATTTCCGGGTCAT | 391 to 368 |
The mutated base is underlined.
Relative to protein S cDNA.44