Adhesion of hematopoietic cell lines to fibronectin, laminin-1, laminin-8, and laminin-10/11
Cell line . | Fibronectin . | Laminin-1 . | Laminin-8 . | Laminin-10/11 . |
---|---|---|---|---|
LP-1 | +/+++ | +/− | +/++ | +++/+++ |
CO | −/++ | −/− | −/− | +/+++ |
BJAB | −/− | −/− | −/− | +++/+ |
NALM/6 | ++/+++ | −/− | −/− | ++/++ |
CA-46 | −/+ | −/− | −/− | −/− |
DAUDI | +/+ | −/− | −/− | −/− |
DG-75 | −/+ | −/− | −/− | +/++ |
KM-3 | +/++ | −/− | −/+ | +/+ |
KG-1 | +/++ | −/− | −/− | +++/++ |
K562 | +/+ | −/− | −/− | +/− |
HEL | +++/+++ | −/− | +/+ | ++/+++ |
NB-4 | −/+ | −/− | −/− | +/++ |
HL-60 | −/+ | −/− | −/− | +++/+ |
Monomac | −/++ | +/− | −/− | +++/+ |
U-937 | −/+++ | −/− | −/+ | ++/++ |
Cell line . | Fibronectin . | Laminin-1 . | Laminin-8 . | Laminin-10/11 . |
---|---|---|---|---|
LP-1 | +/+++ | +/− | +/++ | +++/+++ |
CO | −/++ | −/− | −/− | +/+++ |
BJAB | −/− | −/− | −/− | +++/+ |
NALM/6 | ++/+++ | −/− | −/− | ++/++ |
CA-46 | −/+ | −/− | −/− | −/− |
DAUDI | +/+ | −/− | −/− | −/− |
DG-75 | −/+ | −/− | −/− | +/++ |
KM-3 | +/++ | −/− | −/+ | +/+ |
KG-1 | +/++ | −/− | −/− | +++/++ |
K562 | +/+ | −/− | −/− | +/− |
HEL | +++/+++ | −/− | +/+ | ++/+++ |
NB-4 | −/+ | −/− | −/− | +/++ |
HL-60 | −/+ | −/− | −/− | +++/+ |
Monomac | −/++ | +/− | −/− | +++/+ |
U-937 | −/+++ | −/− | −/+ | ++/++ |
Adhesion was studied without and after treatment of cells with TPA, 5 ng/mL, for 4 hours (without/after TPA). Cell adhesion was measured by spectrophotometer after staining with crystal violet. Adhesion of each cell line was studied at least in triplicate. Adhesion to wells coated with PBS instead of proteins was used as a control.
+ indicates mean absorbance more than 2 times but less than 5 times higher than control; ++, mean absorbance at least 5 times but less than 10 times higher than control; +++, mean absorbance at least 10 times higher than control; –, mean absorbance less than or equal to 2 times higher than control. In each case with mean absorbance more than 2 times higher than control, the difference from the corresponding control value was significant (P < .05).