Effects of activation of wild-type and mutant hIL-3 and hGM-CSF receptors in FDCP-mix cells
| Receptor . | Morphology in rmIL-3 . | Morphology in human cytokine . | Change in Sca-1 expression . | Change in Gr-1 expression . | Change in Mac-1 expression . | Proliferate in culture > 10 d? . |
|---|---|---|---|---|---|---|
| Wild-type hGM Rα, hβc | Blasts | Granulocytes and macrophages | = | ⇑ | ⇑ | No |
| Chimeric hGM/βc, hβc | Blasts | Blasts, EG | ⇑ | = | ⇑ | Yes |
| Wild-type hIL-3 Rα, hβc | Blasts | Blasts | = | = | = | Yes |
| Mutant HGM (M) Rα, hβc | Blasts | Blasts, EG | ⇑ | = | ⇑ | Yes |
| Mutant hIL-3 (M) Rα, hβc | Blasts | Blasts, granulocytes, and macrophages | = | ⇑ | ⇑ | No |
| Receptor . | Morphology in rmIL-3 . | Morphology in human cytokine . | Change in Sca-1 expression . | Change in Gr-1 expression . | Change in Mac-1 expression . | Proliferate in culture > 10 d? . |
|---|---|---|---|---|---|---|
| Wild-type hGM Rα, hβc | Blasts | Granulocytes and macrophages | = | ⇑ | ⇑ | No |
| Chimeric hGM/βc, hβc | Blasts | Blasts, EG | ⇑ | = | ⇑ | Yes |
| Wild-type hIL-3 Rα, hβc | Blasts | Blasts | = | = | = | Yes |
| Mutant HGM (M) Rα, hβc | Blasts | Blasts, EG | ⇑ | = | ⇑ | Yes |
| Mutant hIL-3 (M) Rα, hβc | Blasts | Blasts, granulocytes, and macrophages | = | ⇑ | ⇑ | No |
Data were obtained for the FDCP-mix cell cytokine receptor transfects cultured with either hIL-3 or hGM-CSF for 7 days. The effects on proliferation, morphology, and differentiation markers Sca-1, Gr-1, and Mac-1 are summarized. Differentiation marker expression remained at similar levels (=) increased (⇑) or decreased (⇓).
EG indicates early granulocytes.