Effect of activation of hIL-3 (M) Rα, hβc compared to wild-type hIL-3 Rα, hβcon cell morphology
| Receptor transfect . | Blasts . | Early granulocytes . | Late granulocytes . | Macrophages . |
|---|---|---|---|---|
| Wild-type hIL-3 Rα, hβc | 95.3 ± 0.9 | 4.3 ± 0.7 | 0 ± 0 | 0.3 ± 0.3 |
| hIL-3 (M) Rα, hβc | 25.5 ± 4.5 | 39 ± 0.9 | 35.5 ± 3.5 | 0 ± 0 |
| Receptor transfect . | Blasts . | Early granulocytes . | Late granulocytes . | Macrophages . |
|---|---|---|---|---|
| Wild-type hIL-3 Rα, hβc | 95.3 ± 0.9 | 4.3 ± 0.7 | 0 ± 0 | 0.3 ± 0.3 |
| hIL-3 (M) Rα, hβc | 25.5 ± 4.5 | 39 ± 0.9 | 35.5 ± 3.5 | 0 ± 0 |
Cells were cultured in hIL-3 (1 ng/mL) for 7 days. Morphology was then scored using May-Grünwald-Giemsa–stained cytospin preparations. The results are presented as percentage of total cells and are pooled data from 3 experiments ± SEM. Prior to culture in hIL-3, cell morphology was ≥ 94% blasts.