Effect of activation of hGM/βc chimera with or without hβc on cell morphology
| Receptor transfect . | Blasts . | Early granulocytes . | Late granulocytes . | Macrophages . |
|---|---|---|---|---|
| Wild-type hGM Rα, hβc | 7.5 ± 0.7 | 13 ± 2.8 | 38 ± 4.2 | 41.5 ± 7.8 |
| Chimeric hGM/βc | 67.5 ± 10.7 | 30 ± 12.3 | 1.5 ± 2.1 | 1 ± 1.4 |
| hGM/βc, hβc | 80.5 ± 1 | 18.5 ± 1 | 0 ± 0 | 0 ± 0 |
| Receptor transfect . | Blasts . | Early granulocytes . | Late granulocytes . | Macrophages . |
|---|---|---|---|---|
| Wild-type hGM Rα, hβc | 7.5 ± 0.7 | 13 ± 2.8 | 38 ± 4.2 | 41.5 ± 7.8 |
| Chimeric hGM/βc | 67.5 ± 10.7 | 30 ± 12.3 | 1.5 ± 2.1 | 1 ± 1.4 |
| hGM/βc, hβc | 80.5 ± 1 | 18.5 ± 1 | 0 ± 0 | 0 ± 0 |
Cells were cultured in hGM-CSF (1 ng/mL) for 7 days. Morphology was then assessed using May-Grünwald-Giemsa–stained cytospin preparations. Cell morphology was then scored and the results are presented as percentage of total cells. The results are pooled data from 3 experiments ± SEM. Prior to culture in hGM-CSF cell morphology was ≥ 94% blast.