Antigen receptor gene rearrangements of the leukemias at initial diagnosis and relapse as determined by screening PCR and clonotoypic ASO PCR
| Rearrangement3-150 . | Initial diagnosis . | Relapse . |
|---|---|---|
| Patient 1 | ||
| IGH | VH3-33/DH2-2/J6 | Negative |
| TCRD | Vδ2-Dδ3 | Negative |
| TCRGa | Vγ9-Jγ1.3/2.3 | Negative3-151 |
| TCRGb | Vγ3-Jγ1.3/2.3 | Negative3-151 |
| TCRGc | 10−4 | VγI-Jγ1.3/2.3a3-152 |
| TCRGd | 10−4 | VγI-Jγ1.3/2.3b3-152 |
| Patient 2 | ||
| IGHa | VH1-2/DH2-2/J6 | Negative |
| IGHb | VH3-66/J3 | Negative |
| IGHc | Negative | VH3-13/J5 |
| IGHd | 5 × 10−3 | VH3-33/J4 |
| IGK-KDE | RSS-KDE | RSS-KDE |
| TCRDa | Vδ2-Dδ3 | Negative3-151 |
| TCRDb | 5 × 10−3 | Vδ2-Dδ3 |
| TCRGa | Vγ3-Jγ1.3/2.3 | Negative |
| TCRGb | 5 × 10−3 | Vγ2-Jγ1.3/2.3 |
| TCRGc | Negative | Vγ5-Jγ1.3/2.3 |
| Rearrangement3-150 . | Initial diagnosis . | Relapse . |
|---|---|---|
| Patient 1 | ||
| IGH | VH3-33/DH2-2/J6 | Negative |
| TCRD | Vδ2-Dδ3 | Negative |
| TCRGa | Vγ9-Jγ1.3/2.3 | Negative3-151 |
| TCRGb | Vγ3-Jγ1.3/2.3 | Negative3-151 |
| TCRGc | 10−4 | VγI-Jγ1.3/2.3a3-152 |
| TCRGd | 10−4 | VγI-Jγ1.3/2.3b3-152 |
| Patient 2 | ||
| IGHa | VH1-2/DH2-2/J6 | Negative |
| IGHb | VH3-66/J3 | Negative |
| IGHc | Negative | VH3-13/J5 |
| IGHd | 5 × 10−3 | VH3-33/J4 |
| IGK-KDE | RSS-KDE | RSS-KDE |
| TCRDa | Vδ2-Dδ3 | Negative3-151 |
| TCRDb | 5 × 10−3 | Vδ2-Dδ3 |
| TCRGa | Vγ3-Jγ1.3/2.3 | Negative |
| TCRGb | 5 × 10−3 | Vγ2-Jγ1.3/2.3 |
| TCRGc | Negative | Vγ5-Jγ1.3/2.3 |
Negative indicates negative by clone-specific quantitative PCR (at a sensitivity of 10−4 or more).
The letters a, b, c, d indicate the different rearrangements of one respective antigen receptor gene.
Negative by screening PCR (but not tested by clone-specific quantitative PCR).
Sequences were too short to identify the member of the Vgl family.