Primers used for amplification, sequencing, and quantification of TEL/AML1 breakpoints
| Primer . | Nucleotide sequence . | Gene . | Position* . | PCR/sequencing† . |
|---|---|---|---|---|
| P1 | GCCCTTACATTGGAGAAATGGTCTGGTTCA CG | TEL | 10944-10975 | 1st |
| P2 | AGTGGAGTGCCTTCCGCTAAGCAAG | TEL | 11106-11133 | 2nd |
| P3 | GGATAAAAGACAGGAAGCCGCCCCAG | AML1 | 159009-158984 | 1st |
| P4 | CGTTTCCGAGGTTCCAATATAGAGTCGCAG | AML1 | 158954-158925 | 2nd |
| P5 | CTCTCCGGTGATCCACAATCAACAGTGAGATG | AML1 | 150763-150794 | 1st |
| P6 | CAGTGAGATGACAAAGGAGTCAAGCAGATGGAG | AML1 | 150785-150817 | 2nd |
| P7 | CTTAAACCTGGGCAGAACCCATGTGAAAGACGG | TEL | 14589-14557 | 1st |
| P8 | GACGGGAGGCAAGTTCTAACTGAATCAATGGG | TEL | 14561-14530 | 2nd |
| P9 | CTCAGCCCATCCCCTGGCAATACAGAATG | TEL | 12393-12419 | 1st |
| P10 | AGGAGGCCACTTGTCCTTTTCCAAAACACAATG | TEL | 12519-12551 | 2nd |
| P11 | GGGAGCTGCGCTGTCAAATTGAGTCAGG | AML1 | 144301-144274 | 1st |
| P12 | ACAGTGAACTCACTCTAGGAGACCCGCACG | AML1 | 144227-144198 | 2nd |
| P13 | TCTGCCAGTGTCTTCTGT | TEL | 12689-12706 | Seq |
| P14 | GGCCTGGCACATTTGAAAGG | TEL | 13322-13302 | Seq |
| P15 | CTAGCCCACAGCCAAAAACC | TEL | 14046-14065 | Seq |
| P16 | GAATAGCAACAGCACCTGCCT | TEL | NA | Tq |
| P17 | GCTTAGTAAACATTTTACTCTCAGTGGG | AML1 | NA | Tq |
| P18 | ATAAGGCGGCGTAAGGGTCAGATGTAACC | TEL | NA | Pro |
| P19 | TATCTGTATCTCTTTTTGAAACCATGC | TEL | NA | Tq |
| P20 | GGGACCAATGATTCAGGATATGTAAC | AML1 | NA | Tq |
| P21 | CCACAGTTCCCACAGTCAGAGTCACCAAG | TEL | NA | Pro |
| P22 | GGAGATCCAGGTCTTATTGGTCC | APM-1 | NA | Tq |
| P23 | GCTGAGCGGTATACATAGGC | APM-1 | NA | Tq |
| P24 | CATCGGTGAAACCGGAGTACCCGG | APM-1 | NA | Pro |
| Primer . | Nucleotide sequence . | Gene . | Position* . | PCR/sequencing† . |
|---|---|---|---|---|
| P1 | GCCCTTACATTGGAGAAATGGTCTGGTTCA CG | TEL | 10944-10975 | 1st |
| P2 | AGTGGAGTGCCTTCCGCTAAGCAAG | TEL | 11106-11133 | 2nd |
| P3 | GGATAAAAGACAGGAAGCCGCCCCAG | AML1 | 159009-158984 | 1st |
| P4 | CGTTTCCGAGGTTCCAATATAGAGTCGCAG | AML1 | 158954-158925 | 2nd |
| P5 | CTCTCCGGTGATCCACAATCAACAGTGAGATG | AML1 | 150763-150794 | 1st |
| P6 | CAGTGAGATGACAAAGGAGTCAAGCAGATGGAG | AML1 | 150785-150817 | 2nd |
| P7 | CTTAAACCTGGGCAGAACCCATGTGAAAGACGG | TEL | 14589-14557 | 1st |
| P8 | GACGGGAGGCAAGTTCTAACTGAATCAATGGG | TEL | 14561-14530 | 2nd |
| P9 | CTCAGCCCATCCCCTGGCAATACAGAATG | TEL | 12393-12419 | 1st |
| P10 | AGGAGGCCACTTGTCCTTTTCCAAAACACAATG | TEL | 12519-12551 | 2nd |
| P11 | GGGAGCTGCGCTGTCAAATTGAGTCAGG | AML1 | 144301-144274 | 1st |
| P12 | ACAGTGAACTCACTCTAGGAGACCCGCACG | AML1 | 144227-144198 | 2nd |
| P13 | TCTGCCAGTGTCTTCTGT | TEL | 12689-12706 | Seq |
| P14 | GGCCTGGCACATTTGAAAGG | TEL | 13322-13302 | Seq |
| P15 | CTAGCCCACAGCCAAAAACC | TEL | 14046-14065 | Seq |
| P16 | GAATAGCAACAGCACCTGCCT | TEL | NA | Tq |
| P17 | GCTTAGTAAACATTTTACTCTCAGTGGG | AML1 | NA | Tq |
| P18 | ATAAGGCGGCGTAAGGGTCAGATGTAACC | TEL | NA | Pro |
| P19 | TATCTGTATCTCTTTTTGAAACCATGC | TEL | NA | Tq |
| P20 | GGGACCAATGATTCAGGATATGTAAC | AML1 | NA | Tq |
| P21 | CCACAGTTCCCACAGTCAGAGTCACCAAG | TEL | NA | Pro |
| P22 | GGAGATCCAGGTCTTATTGGTCC | APM-1 | NA | Tq |
| P23 | GCTGAGCGGTATACATAGGC | APM-1 | NA | Tq |
| P24 | CATCGGTGAAACCGGAGTACCCGG | APM-1 | NA | Pro |
In all TEL primers, numbering starts with the first nucleotide of exon 5; in the case of AML1, numbers start with the first nucleotide of exon 1.
Indicates the use of the primer either for PCR round 1 and round 2, sequencing (Seq), as TaqMan primers (Tq), or as TaqMan probes (Pro).
NA indicates not applicable.