Comparable expansion of HLA-A*0201-restricted influenza MP-specific CD8+ T by pDCs and CD11c+ DCs
| Experiment no. . | Unstimulated T cells . | Stimulated T cells . | |||
|---|---|---|---|---|---|
| MP IL-3 pDCs . | Flu pDCs . | MP CD11c+ DCs . | Flu CD11c+ DCs . | ||
| 1 | 0.09 | 2.68 | 2.22 | 3.35 | 1.92 |
| 2 | 0.07 | 0.82 | 0.63 | 1.17 | 0.69 |
| 3 | 0.05 | 1.19 | 3.87 | 4.64 | 3.62 |
| 4 | 0.07 | 0.49 | 0.78 | 1.15 | 0.88 |
| 5 | 0.03 | 0.08 | 0.36 | 0.08 | 0.18 |
| Experiment no. . | Unstimulated T cells . | Stimulated T cells . | |||
|---|---|---|---|---|---|
| MP IL-3 pDCs . | Flu pDCs . | MP CD11c+ DCs . | Flu CD11c+ DCs . | ||
| 1 | 0.09 | 2.68 | 2.22 | 3.35 | 1.92 |
| 2 | 0.07 | 0.82 | 0.63 | 1.17 | 0.69 |
| 3 | 0.05 | 1.19 | 3.87 | 4.64 | 3.62 |
| 4 | 0.07 | 0.49 | 0.78 | 1.15 | 0.88 |
| 5 | 0.03 | 0.08 | 0.36 | 0.08 | 0.18 |
T cells, pDCs, and CD11c+ DCs were purified from HLA-A*0201+ PBMCs. T cells were cocultured with pDCs or CD11c+ DCs infected with influenza virus or pulsed with influenza MP(58-66) peptide. Then, 7 days later, the presence of HLA-A*0201-restricted MP-specific CD8+ T cells in the cultures and in a frozen sample of unstimulated T cells was measured by flow cytometry using FITC-conjugated CD8 antibody and PE-conjugated MP(58-66)/HLA-A*0201 tetrameric complexes. Results were obtained with 5 different donors and are expressed as percent of MP tetramer+/CD8+ cells.