Table 4. Tacrolimus as a single agent... | American Society of Hematology

Table 4.

Tacrolimus as a single agent facilitates donor engraftment in mice conditioned with 200 or 100 cGy TBI

Reagent	No. chimeric	% donor	Range
TBI 200 cGy
Hamster IgG	0/10	0 ± 0	NA
Anti-CD40L	10/10^4-150	69 ± 5^4-150	64 -76
Tacrolimus	5/5^4-150	50 ± 4^4-150,^4-151	46 -56
Anti-CD40L + tacrolimus	9/9^4-150	44 ± 6^4-150,^4-151	39 -55
TBI 100 cGy
Hamster IgG	0/10	0 ± 0	NA
Anti-CD40L	10/10^4-150	50 ± 6^4-150	40 -56
Tacrolimus	10/10^4-150	44 ± 7^4-150	33 -53
Anti-CD40L + tacrolimus	9/9^4-150	42 ± 7^4-150,^4-151	28 -50

Reagent	No. chimeric	% donor	Range
TBI 200 cGy
Hamster IgG	0/10	0 ± 0	NA
Anti-CD40L	10/10^4-150	69 ± 5^4-150	64 -76
Tacrolimus	5/5^4-150	50 ± 4^4-150,^4-151	46 -56
Anti-CD40L + tacrolimus	9/9^4-150	44 ± 6^4-150,^4-151	39 -55
TBI 100 cGy
Hamster IgG	0/10	0 ± 0	NA
Anti-CD40L	10/10^4-150	50 ± 6^4-150	40 -56
Tacrolimus	10/10^4-150	44 ± 7^4-150	33 -53
Anti-CD40L + tacrolimus	9/9^4-150	42 ± 7^4-150,^4-151	28 -50

B6 mice were irradiated with 200 or 100 cGy TBI as indicated on day − 1 and infused with 40 × 10⁶ BALB/c BM on day 0. Reagents were administered from day − 1 through day 14. PBLs were typed for percentage of donor-host at 3.5 months after BMT. Chimeric is defined as more than 3% donor PBLs. Percentage donor is defined as average percentage donor cells of all mice in the group ± 1 SD. Range indicates lowest to highest level of percentage donor cells in engrafted mice. NA indicates not applicable.

F4-150

P < .001 compared with hamster IgG.

F4-151

P ≤ .02 compared with anti-CD40L as a single agent.

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