Anti-CD40L alone or in combination with sirolimus or CsA results in high levels of donor chimerism in mice conditioned with 200 cGy TBI
| Reagent . | No. chimeric . | % donor . | Range . |
|---|---|---|---|
| Experiment 1 | |||
| Hamster IgG | 0/10 | 0 ± 0 | NA |
| Anti-CD40L | 10/10* | 69 ± 5* | 64 -76 |
| Sirolimus | 0/10† | 0 ± 0† | NA |
| Anti-CD40L + sirolimus | 9/9* | 75 ± 6*,† | 70 -83 |
| Experiment 2 | |||
| Hamster IgG | 0/15 | 0 ± 0 | NA |
| Anti-CD40L | 9/10* | 64 ± 24* | 61 -85 |
| CsA | 3/9*,† | 6 ± 14† | 3 -43 |
| Anti-CD40L + CsA | 17/17* | 62 ± 13* | 28 -78 |
| Reagent . | No. chimeric . | % donor . | Range . |
|---|---|---|---|
| Experiment 1 | |||
| Hamster IgG | 0/10 | 0 ± 0 | NA |
| Anti-CD40L | 10/10* | 69 ± 5* | 64 -76 |
| Sirolimus | 0/10† | 0 ± 0† | NA |
| Anti-CD40L + sirolimus | 9/9* | 75 ± 6*,† | 70 -83 |
| Experiment 2 | |||
| Hamster IgG | 0/15 | 0 ± 0 | NA |
| Anti-CD40L | 9/10* | 64 ± 24* | 61 -85 |
| CsA | 3/9*,† | 6 ± 14† | 3 -43 |
| Anti-CD40L + CsA | 17/17* | 62 ± 13* | 28 -78 |
B6 mice were irradiated with 200 cGy TBI on day − 1 and infused with 40 × 106 BALB/c BM on day 0. Reagents were administered from day − 1 through day 14. PBLs were typed for percentage of donor-host at 3.5 months after BMT. Chimeric is defined as more than 3% donor PBLs. Percentage donor is defined as average percentage donor cells of all mice in the group ± 1 SD. Range indicates lowest to highest level of percentage donor cells in engrafted mice. NA indicates not applicable.
P ≤ .017 compared with hamster IgG.
P ≤ .011 compared with anti-CD40L as a single agent.