α1-3-fucosyltransferase activities in human and murine leukocytes
Cells . | α1-3-fucosyltransferase activity (pmol/h/mg) . | |
---|---|---|
GP-4 . | GP-5 . | |
Murine leukocytes | 4.9 ± 0.5 | 8.6 ± 1.7 |
Murine neutrophils | 6.4 ± 1.9 | 13.7 ± 1.7 |
WEHI-3 cells | 6.0 ± 0.5 | 5.6 ± 0.6 |
Human leukocytes | 7.3 ± 0.2 | 12.9 ± 5.1 |
Human neutrophils | 6.7 ± 1.0 | 27.9 ± 5.6 |
HL-60 cells | 125.0 ± 3.0 | 254.0 ± 20.8 |
Cells . | α1-3-fucosyltransferase activity (pmol/h/mg) . | |
---|---|---|
GP-4 . | GP-5 . | |
Murine leukocytes | 4.9 ± 0.5 | 8.6 ± 1.7 |
Murine neutrophils | 6.4 ± 1.9 | 13.7 ± 1.7 |
WEHI-3 cells | 6.0 ± 0.5 | 5.6 ± 0.6 |
Human leukocytes | 7.3 ± 0.2 | 12.9 ± 5.1 |
Human neutrophils | 6.7 ± 1.0 | 27.9 ± 5.6 |
HL-60 cells | 125.0 ± 3.0 | 254.0 ± 20.8 |
Enzyme activities were measured using the nonsialylated glycopeptide GP-4 or the sialylated glycopeptide GP-5 as acceptor. Results are expressed as the mean ± range of 2 determinations for HL-60 cells and the mean ± SD of 3 determinations for all other cells.