Table 1.

α1-3-fucosyltransferase activities in human and murine leukocytes

Cellsα1-3-fucosyltransferase activity (pmol/h/mg)
GP-4GP-5
Murine leukocytes 4.9  ±  0.5 8.6  ±  1.7  
Murine neutrophils 6.4  ±  1.9 13.7  ±  1.7  
WEHI-3 cells 6.0  ±  0.5 5.6  ±  0.6  
Human leukocytes 7.3  ±  0.2 12.9  ±  5.1  
Human neutrophils 6.7  ±  1.0 27.9  ±  5.6  
HL-60 cells 125.0  ±  3.0 254.0  ±  20.8 
Cellsα1-3-fucosyltransferase activity (pmol/h/mg)
GP-4GP-5
Murine leukocytes 4.9  ±  0.5 8.6  ±  1.7  
Murine neutrophils 6.4  ±  1.9 13.7  ±  1.7  
WEHI-3 cells 6.0  ±  0.5 5.6  ±  0.6  
Human leukocytes 7.3  ±  0.2 12.9  ±  5.1  
Human neutrophils 6.7  ±  1.0 27.9  ±  5.6  
HL-60 cells 125.0  ±  3.0 254.0  ±  20.8 

Enzyme activities were measured using the nonsialylated glycopeptide GP-4 or the sialylated glycopeptide GP-5 as acceptor. Results are expressed as the mean ± range of 2 determinations for HL-60 cells and the mean ± SD of 3 determinations for all other cells.

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