Bone marrow engraftment by transduced primary cord blood cells
| CB CD34+ cell dose . | Engraftment, % CD45+ . | 45+/GFP+ cells, % . | ||||
|---|---|---|---|---|---|---|
| 15 × 106 TU/mL, MOI 15 | ||||||
| 100 000 | 10 | 11.6 | 8.3 | 10.3 | 9.8 | 10.6 |
| 200 000 | 18 | 19.1 | 13.3 | 12.5 | 15.4 | 8.9 |
| 500 000 | 36 | 40.3 | 42 | 10.6 | 15.4 | 9.6 |
| 50 × 106 TU/mL, MOI 50 | ||||||
| 100 000 | 15.1 | 8.7 | 10.6 | 12.4 | 15.8 | 11.7 |
| 200 000 | 18.4 | 19.6 | 20.8 | 14.3 | 16.2 | 12.4 |
| 500 000 | 41.3 | 24.6 | 48.4 | 12.6 | 15.4 | 18.8 |
| CB CD34+ cell dose . | Engraftment, % CD45+ . | 45+/GFP+ cells, % . | ||||
|---|---|---|---|---|---|---|
| 15 × 106 TU/mL, MOI 15 | ||||||
| 100 000 | 10 | 11.6 | 8.3 | 10.3 | 9.8 | 10.6 |
| 200 000 | 18 | 19.1 | 13.3 | 12.5 | 15.4 | 8.9 |
| 500 000 | 36 | 40.3 | 42 | 10.6 | 15.4 | 9.6 |
| 50 × 106 TU/mL, MOI 50 | ||||||
| 100 000 | 15.1 | 8.7 | 10.6 | 12.4 | 15.8 | 11.7 |
| 200 000 | 18.4 | 19.6 | 20.8 | 14.3 | 16.2 | 12.4 |
| 500 000 | 41.3 | 24.6 | 48.4 | 12.6 | 15.4 | 18.8 |
Results from 6 different experiments are shown. Values show the proportion of human CD45+ and CD45+/GFP+ cells in the BM of 3 NOD/SCID mice, each inoculated with the indicated cell dose. Cells were prestimulated for 24 to 48 hours in the presence of FL, TPO, SCF, and IL6 and subsequently incubated for 12 hours in the presence of the lentiviral vector at the indicated concentration.