TRAIL-sensitivity and cell-surface expression of TRAIL receptors in Ph1-positive leukemia cell lines
| Cell line . | 3H-thymidine uptake, cpm* . | % inhibition by TRAIL† . | Expression of TRAIL receptors, RFI‡ . | ||||
|---|---|---|---|---|---|---|---|
| Control . | TRAIL . | DR4 . | DR5 . | DcR1 . | DcR2 . | ||
| CML-BC-derived | |||||||
| KOPM28 | 103 712 ± 1849 | 6 000 ± 54 | 95 | 1.0 | 3.4 | 1.1 | 1.0 |
| KOPM53 | 200 509 ± 9527 | 165 725 ± 8340 | 18 | 1.1 | 1.0 | 1.0 | 1.0 |
| KOPN55bi | 11 705 ± 1730 | 9 954 ± 398 | 10 | 1.0 | 1.3 | 1.1 | 1.1 |
| Nalm1 | 28 920 ± 621 | 167 ± 14 | 99 | 1.7 | 2.1 | 1.0 | 1.0 |
| K562 | 12 099 ± 213 | 10 034 ± 61 | 20 | 1.5 | 5.0 | 1.0 | 1.0 |
| Ph1-positive AL-derived | |||||||
| KOPM30 | 29 182 ± 1796 | 7 055 ± 368 | 75 | 1.6 | 3.2 | 1.0 | 1.0 |
| KOPN30bi | 114 528 ± 4592 | 116 241 ± 2666 | −1 | 1.0 | 1.7 | 1.1 | 1.1 |
| KOPN57bi | 40 975 ± 2021 | 35 681 ± 2001 | 5 | 1.0 | 1.3 | 1.0 | 1.1 |
| KOPN66bi | 75 603 ± 1175 | 9 031 ± 157 | 85 | 1.5 | 2.6 | 1.0 | 1.0 |
| KOPN72bi | 112 855 ± 5916 | 68 357 ± 4469 | 52 | 1.7 | 1.1 | 1.0 | 1.0 |
| YAMN73 | 36 437 ± 716 | 20 553 ± 498 | 45 | 1.2 | 2.9 | 1.2 | 1.0 |
| YAMN91 | 27 788 ± 1529 | 1 062 ± 79 | 96 | 1.0 | 3.4 | 1.1 | 1.0 |
| Cell line . | 3H-thymidine uptake, cpm* . | % inhibition by TRAIL† . | Expression of TRAIL receptors, RFI‡ . | ||||
|---|---|---|---|---|---|---|---|
| Control . | TRAIL . | DR4 . | DR5 . | DcR1 . | DcR2 . | ||
| CML-BC-derived | |||||||
| KOPM28 | 103 712 ± 1849 | 6 000 ± 54 | 95 | 1.0 | 3.4 | 1.1 | 1.0 |
| KOPM53 | 200 509 ± 9527 | 165 725 ± 8340 | 18 | 1.1 | 1.0 | 1.0 | 1.0 |
| KOPN55bi | 11 705 ± 1730 | 9 954 ± 398 | 10 | 1.0 | 1.3 | 1.1 | 1.1 |
| Nalm1 | 28 920 ± 621 | 167 ± 14 | 99 | 1.7 | 2.1 | 1.0 | 1.0 |
| K562 | 12 099 ± 213 | 10 034 ± 61 | 20 | 1.5 | 5.0 | 1.0 | 1.0 |
| Ph1-positive AL-derived | |||||||
| KOPM30 | 29 182 ± 1796 | 7 055 ± 368 | 75 | 1.6 | 3.2 | 1.0 | 1.0 |
| KOPN30bi | 114 528 ± 4592 | 116 241 ± 2666 | −1 | 1.0 | 1.7 | 1.1 | 1.1 |
| KOPN57bi | 40 975 ± 2021 | 35 681 ± 2001 | 5 | 1.0 | 1.3 | 1.0 | 1.1 |
| KOPN66bi | 75 603 ± 1175 | 9 031 ± 157 | 85 | 1.5 | 2.6 | 1.0 | 1.0 |
| KOPN72bi | 112 855 ± 5916 | 68 357 ± 4469 | 52 | 1.7 | 1.1 | 1.0 | 1.0 |
| YAMN73 | 36 437 ± 716 | 20 553 ± 498 | 45 | 1.2 | 2.9 | 1.2 | 1.0 |
| YAMN91 | 27 788 ± 1529 | 1 062 ± 79 | 96 | 1.0 | 3.4 | 1.1 | 1.0 |
Data are shown as means ± SE.
% inhibition by TRAIL was determined by the 3H-thymidine uptake assay in the presence or absence of 100 ng/mL rhsTRAIL as described in “Materials and methods.”
RFI was determined by the ratio of mean fluorescence intensity for specific staining to that for control staining.