Table 1. Characteristics and sensitivity... | American Society of Hematology

Table 1.

Characteristics and sensitivity to FasL in Ph1-positive leukemia cell lines

Cell line	BCR-ABL	Linage	³H-thymidine uptake, cpm^*		% inhibition by FasL^†	Expression of Fas, RFI^‡
Cell line	BCR-ABL	Linage	Control	FasL	% inhibition by FasL^†	Expression of Fas, RFI^‡
CML-BC-derived
KOPM28	p210	Myeloid	226 181 ± 2256	244 361 ± 1326	−8	3.0
KOPM53	p210	Myeloid	178 218 ± 1645	174 291 ± 1364	2	2.4
KOPN55bi	p210	Lymphoid	8 184 ± 340	7 969 ± 493	4	1.0
Nalm1	p210	Lymphoid	40 925 ± 269	36 365 ± 1361	12	1.9
K562	p210	Myeloid	9 611 ± 110	9 906 ± 295	−3	1.0
Ph1-positive AL-derived
KOPM30	p190	Myeloid	10 849 ± 141	7 511 ± 375	30	2.2
KOPN30bi	p190	Lymphoid	186 993 ± 4663	180 406 ± 3438	4	1.1
KOPN57bi	p190	Lymphoid	90 605 ± 2044	86 368 ± 5687	4	1.5
KOPN66bi	p190	Lymphoid	117 272 ± 2663	114 914 ± 2197	2	1.3
KOPN72bi	p190	Lymphoid	145 957 ± 3415	140 332 ± 4242	4	1.2
YAMN73	p203^1-153	Lymphoid	60 397 ± 991	59 396 ± 1840	2	1.0
YAMN91	p190	Lymphoid	13 884 ± 246	14 143 ± 167	−2	1.3

Cell line	BCR-ABL	Linage	³H-thymidine uptake, cpm^*		% inhibition by FasL^†	Expression of Fas, RFI^‡
Cell line	BCR-ABL	Linage	Control	FasL	% inhibition by FasL^†	Expression of Fas, RFI^‡
CML-BC-derived
KOPM28	p210	Myeloid	226 181 ± 2256	244 361 ± 1326	−8	3.0
KOPM53	p210	Myeloid	178 218 ± 1645	174 291 ± 1364	2	2.4
KOPN55bi	p210	Lymphoid	8 184 ± 340	7 969 ± 493	4	1.0
Nalm1	p210	Lymphoid	40 925 ± 269	36 365 ± 1361	12	1.9
K562	p210	Myeloid	9 611 ± 110	9 906 ± 295	−3	1.0
Ph1-positive AL-derived
KOPM30	p190	Myeloid	10 849 ± 141	7 511 ± 375	30	2.2
KOPN30bi	p190	Lymphoid	186 993 ± 4663	180 406 ± 3438	4	1.1
KOPN57bi	p190	Lymphoid	90 605 ± 2044	86 368 ± 5687	4	1.5
KOPN66bi	p190	Lymphoid	117 272 ± 2663	114 914 ± 2197	2	1.3
KOPN72bi	p190	Lymphoid	145 957 ± 3415	140 332 ± 4242	4	1.2
YAMN73	p203^1-153	Lymphoid	60 397 ± 991	59 396 ± 1840	2	1.0
YAMN91	p190	Lymphoid	13 884 ± 246	14 143 ± 167	−2	1.3

Data are shown as means ± SE of triplicate cultures.

†

% inhibition by FasL was determined by the ³H-thymidine uptake assay in the presence or absence of 100 ng/mL rhsFasL as described in “Materials and methods.”

‡

Relative fluorescence intensity (RFI) was determined by the ratio of mean fluorescence intensity for specific staining to that for control staining.

F1-153

p203 corresponds to the Abl exon2-spliced BCR-ABL protein.

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