Confirmation of chimeric antibodies' isotypes
| Chimeric antibodies . | Human Ig–specific antibodies . | |||||
|---|---|---|---|---|---|---|
| IgA1 . | IgA2 . | IgG1 . | IgG2 . | IgG3 . | IgG4 . | |
| chF3.3 IgA1 | 7.2 | 1.3 | 1.2 | 1.0 | 1.0 | 1.0 |
| chF3.3 IgA2 | 1.0 | 4.5 | 1.2 | 1.0 | 1.0 | 1.0 |
| chF3.3 IgG1 | 1.0 | 1.1 | 11.4 | 1.0 | 1.2 | 1.0 |
| chF3.3 IgG2 | 1.0 | 1.2 | 1.3 | 3.6 | 1.0 | 1.0 |
| chF3.3 IgG3 | 1.0 | 1.1 | 1.2 | 1.0 | 13.3 | 1.0 |
| chF3.3 IgG4 | 1.0 | 1.2 | 1.3 | 1.0 | 1.0 | 7.1 |
| Chimeric antibodies . | Human Ig–specific antibodies . | |||||
|---|---|---|---|---|---|---|
| IgA1 . | IgA2 . | IgG1 . | IgG2 . | IgG3 . | IgG4 . | |
| chF3.3 IgA1 | 7.2 | 1.3 | 1.2 | 1.0 | 1.0 | 1.0 |
| chF3.3 IgA2 | 1.0 | 4.5 | 1.2 | 1.0 | 1.0 | 1.0 |
| chF3.3 IgG1 | 1.0 | 1.1 | 11.4 | 1.0 | 1.2 | 1.0 |
| chF3.3 IgG2 | 1.0 | 1.2 | 1.3 | 3.6 | 1.0 | 1.0 |
| chF3.3 IgG3 | 1.0 | 1.1 | 1.2 | 1.0 | 13.3 | 1.0 |
| chF3.3 IgG4 | 1.0 | 1.2 | 1.3 | 1.0 | 1.0 | 7.1 |
Isotypes of chimeric F3.3 antibodies were confirmed by indirect immunofluorescence of HLA class II–transfected L66 cells using human IgA1-, IgA2-, IgG1-, IgG2-, IgG3-, or IgG4-specific antibodies. FITC-labeled antimouse IgG antibody was used for staining. Data from 1 of 2 experiments are presented as RFIs, calculated as the ratio of mean linear fluorescence intensity of relevant to irrelevant, isotype-controlled antibodies.