Gene array analysis of SCF-stimulated eosinophils
| Gene . | Fold expression over control . | ||
|---|---|---|---|
| 1 h* . | 6 h . | 18 h . | |
| Chemokines | |||
| CCL2/MCP-1 | 2.1-3.8 | 0.849 | 0.887 |
| CCL4/MIP-1β | 2.0-4.0 | 0.252 | 1.071 |
| CCL5/RANTES | 120-235 | 0.973 | 0.895 |
| CCL17/TARC | 2.2-25 | 0.79 | 0.84 |
| CCL21/SLC | 7.01-24.9 | 0.81 | 1.30 |
| CL22/MDC | 2.01-2.8 | 0.49 | 1.55 |
| CCR2 | 3.2-160 | 0.78 | 0.269 |
| CxCR3 | 2.69-3.5 | 0.65 | 2.48 |
| CxCR5 | 3.8-5.7 | 1.02 | 0.56 |
| IL receptors | |||
| IL-2RG | 6.1-17.9 | 0.79 | 1.99 |
| IL-11RA | 3.26-3.9 | 0.417 | 1.04 |
| Growth factors | |||
| FGF5 | 3.07-3.5 | 0.91 | 3.65 |
| FGF7 (KGF) | 2.2-4.1 | 0.634 | 1.84 |
| PDGFA | 8.5-25.6 | 1.27 | 0.41 |
| TGFβ3 | 4.7-9.2 | 0.70 | 0.69 |
| Signal transduction and promoter | |||
| MAPK4 | 3.4-13.7 | 0.58 | 0.73 |
| MAPK11 | 2.6-3.5 | 1.54 | 2.89 |
| STAT5A | 3.9-10.8 | 0.68 | 2.19 |
| STAT6 | 12.2-175.6 | 0.98 | 0.84 |
| Adhesion molecules | |||
| ITGA2 (VLA4, CD49B) | 3.7-8.1 | 0.94 | 1.07 |
| ITGAM (CD11B) | 2.02-3.5 | 0.87 | 2.935 |
| ITGB1 (CD29) | 3.5-6.8 | 0.46 | 1.76 |
| ITGB2 (CD18) | 2.4-2.6 | 0.63 | 1.67 |
| ITGB4 (β4) | 6.0-15.1 | 0.87 | 2.12 |
| Gene . | Fold expression over control . | ||
|---|---|---|---|
| 1 h* . | 6 h . | 18 h . | |
| Chemokines | |||
| CCL2/MCP-1 | 2.1-3.8 | 0.849 | 0.887 |
| CCL4/MIP-1β | 2.0-4.0 | 0.252 | 1.071 |
| CCL5/RANTES | 120-235 | 0.973 | 0.895 |
| CCL17/TARC | 2.2-25 | 0.79 | 0.84 |
| CCL21/SLC | 7.01-24.9 | 0.81 | 1.30 |
| CL22/MDC | 2.01-2.8 | 0.49 | 1.55 |
| CCR2 | 3.2-160 | 0.78 | 0.269 |
| CxCR3 | 2.69-3.5 | 0.65 | 2.48 |
| CxCR5 | 3.8-5.7 | 1.02 | 0.56 |
| IL receptors | |||
| IL-2RG | 6.1-17.9 | 0.79 | 1.99 |
| IL-11RA | 3.26-3.9 | 0.417 | 1.04 |
| Growth factors | |||
| FGF5 | 3.07-3.5 | 0.91 | 3.65 |
| FGF7 (KGF) | 2.2-4.1 | 0.634 | 1.84 |
| PDGFA | 8.5-25.6 | 1.27 | 0.41 |
| TGFβ3 | 4.7-9.2 | 0.70 | 0.69 |
| Signal transduction and promoter | |||
| MAPK4 | 3.4-13.7 | 0.58 | 0.73 |
| MAPK11 | 2.6-3.5 | 1.54 | 2.89 |
| STAT5A | 3.9-10.8 | 0.68 | 2.19 |
| STAT6 | 12.2-175.6 | 0.98 | 0.84 |
| Adhesion molecules | |||
| ITGA2 (VLA4, CD49B) | 3.7-8.1 | 0.94 | 1.07 |
| ITGAM (CD11B) | 2.02-3.5 | 0.87 | 2.935 |
| ITGB1 (CD29) | 3.5-6.8 | 0.46 | 1.76 |
| ITGB2 (CD18) | 2.4-2.6 | 0.63 | 1.67 |
| ITGB4 (β4) | 6.0-15.1 | 0.87 | 2.12 |
The fold increase over control eosinophils was calculated from repeat experiments where eosinophils were activated with SCF (100 ng/mL) for 1, 6, or 18 hours. The 1-hour time point was run a total of 4 times, whereas the 6- and 18-hour time points were analyzed only twice. The range of readings is given for only the 1-hour time point and the mean of the repeats is presented for the 6- and 18-hour time points.
CCL2 indicates C-C chemokine receptor ligand 2; MCP-1, membrane-associated cysteine protease–1; RANTES, regulated and normal T-cell expressed and presumably secreted; TARC, thymus and activation-regulated chemokine; SLC, secondary lymphoid-tissue chemokine; PDGFA, platelet-derived growth factor; MAPK4, mitogen-activated protein kinase; STAT5A, signal transducer and activator of transcription 5A; IGTA2, integrin A2; and VLA4, very-late activation protein A4.
All values at 1 hour were P < .05.