Table 1.

Genes modulated by 2ME2 in MM cells

Gene nameGenBank no.2ME2 (fold change over control)
6 hr12 hr24 hr48 hr72 hr
Growth/proliferation and cell adhesion       
 Interleukin 2 receptor, γ D11086 −1.8 −1.8 −2.1 −3.0 −2.5 
 BTG family, member 2 U72649 −1.8 −1.9 −2.4 −2.9 −1.9  
 Small inducible cytokine A3 D90144 −1.5 −1.4 −1.7 −2.4 1.5  
 Rho GDP dissociation inhibitor (GDI) X69549 −2.0 −1.9 −1.5 −1.6 −2.5 
 G-protein coupled receptor AF091890 1.3 2.6 1.2 1.3 1.3  
 Mucin 1, transmembrane X52228 2.1 1.5 1.6 1.5 1.8 
 Integrin, β 5 M35011 1.5 2.5 1.5 1.6 1.3 
 LPS-induced TNF-α factor AF010312 −3.1 −3.6 −2.8 −2.9 −2.3 
 TNF-α–induced protein 3 M59465 −2.3 −2.0 −2.8 −3.0 −2.6  
 Antigen identified by monoclonal antibody Ki-67 X65550 −2.6 −2.3 −1.2 −1.7 −2.1 
 Farnesyl diphosphate synthase D14697 −2.3 −2.2 −1.8 −1.7 −1.7 
Apoptosis/survival/drug resistance       
 Proline dehydrogenase (oxidase) 1 U82381 2.8 2.1 2.4 2.1 2.2  
 Creatine kinase X15334 2.9 1.8 1.5 1.3 1.4  
 Poly (ADP-ribose) polymerase J03473 −1.4 −1.7 −1.7 −1.6 −1.9 
 Crystalin, β B1 U35340 2.5 2.1 1.3 1.7 1.5 
 C-myc-P64 M13929 2.5 2.4 1.8 1.6 1.1 
 Casein kinase 1, epsilon L37043 −2.1 −1.7 −1.8 −1.7 −1.9 
 NM23/nonmetastatic cells 4 Y07604 −2.1 −1.9 −2.2 −2.3 −2.8 
 C-terminal binding protein 1 U37408 −2.2 −2.5 −2.4 −3.4 −2.8  
 Pyruvate kinase M26252 −3.4 −4.6 −3.1 −5.0 −6.1 
 CDC28 protein kinase 2 X54942 −1.6 −1.6 1.5 2.5 1.4  
Protein tyrosine phosphatase, nonreceptor type 6 X62055 −3.5 −3.3 −2.5 −2.8 −2.3  
 Lectin, galactoside-binding, soluble, 1 (galactin 1) AI535946 −2.5 −2.8 −3.1 −1.9 −2.3 
 V-raf-1 murine leukemia viral oncogene homolog 1 X06409 −2.5 −3.1 −2.5 −2.2 −1.8  
 Myeloid cell leukemia sequence 1 (BCL2 related) L08246 −2.2 −2.1 −2.2 −2.4 −1.9 
 Adrenomedullin D14874 −2.4 −2.6 −3.2 −4.8 −6.4 
 X-box binding protein 1 Z93930 −1.9 −2.4 −2.5 −3.2 −1.8 
 Pim-2oncogene U77735 −1.5 −1.3 −1.4 −2.3 −2.0 
Endoplasmic reticulum and translation       
 Eukaryotic translation initiation factor 5A AI074025 2.0 1.6 2.1 1.5 1.4 
 Eukaryotic translation initiation factor 4B X55733 −2.8 −2.7 −2.8 −3.1 −2.6 
 Eukaryotic translation elongation factor 1 L41498 −2.0 −2.3 −2.2 −2.1 −1.0 
 Eukaryotic translation initiation factor 3 U94855 −2.3 −2.3 −1.6 −1.5 −1.7  
 TAP binding protein (tapasin) AF029750 −2.2 −2.8 −2.6 −3.0 −1.9 
 Tumor rejection antigen (gp96) X15187 −1.9 −2.4 −2.4 −2.3 −1.4 
 Endoplasmic reticulum stress inducible AF055001 −1.5 −2.2 −2.4 −2.0 1.2 
Cytoskeleton/structural       
 Suppression of tumorigenicity 5 U15131 2.0 2.1 1.6 1.7 1.6 
 Tubulin, β, 2 X02344 −1.9 −2.4 −1.3 −1.5 −2.4 
 Actin-related protein 2/3 complex AF006088 −1.2 −1.5 −2.6 −1.3 −1.5 
 Vimentin Z19554 −2.1 −2.9 −1.7 −1.4 −1.4 
 Lymphocyte specific protein 1 M33552 −4.0 −4.2 −2.7 −2.6 −2.2  
 Adducin 1 (α) X58141 −1.7 −1.8 −2.2 −2.3 −1.9 
Ubiquitins and metabolism       
 Nucleosome assembly protein 1-like 1 W26056 −1.9 −2.5 −2.3 −2.3 −2.0 
 γ-glutamyltransferase 2 M30474 −2.0 −2.5 −1.4 −1.8 −1.3 
 Phosphomannomutase 2 U85773 2.4 1.9 1.4 1.7 1.5 
 Ubiquitin-conjugating enzyme E2M AF075599 2.2 2.1 2.6 2.4 2.0  
 Karyopherin α 2 (RAG) U28386 −1.1 −1.2 1.2 1.2 −2.1 
 Solute carrier family 25 J03592 −2.6 −2.7 −2.1 −1.9 −1.5 
 Cytochrome c oxidase subunit VIa polypeptide 1 AI540925 −1.6 −1.8 −1.3 −1.2 −1.3 
 Ubiquitin-conjugating enzyme E2G 2 AF032456 1.7 1.7 1.1 1.8 1.1 
 Ubiquitin-conjugating enzyme E2E 3 AB017644 −1.6 −1.6 −1.8 −1.6 −1.9 
 Splicing factor, arginine/serine-rich 6 AL031681 2.0 1.5 −1.0 −1.2 −1.1 
 Leukotriene A4 hydrolase J03459 −2.4 −2.4 −2.0 −2.8 −2.1 
 Ubiquitin-activating enzyme E1 M58028 −2.2 −2.8 −2.2 −2.6 −2.0  
 FK506 binding protein 1A (12 kDa) V00599 −2.1 −1.9 −1.5 −2.9 −4.5  
 RNA binding motif protein 3 U28686 1.8 2.1 1.6 1.5 1.4 
Gene nameGenBank no.2ME2 (fold change over control)
6 hr12 hr24 hr48 hr72 hr
Growth/proliferation and cell adhesion       
 Interleukin 2 receptor, γ D11086 −1.8 −1.8 −2.1 −3.0 −2.5 
 BTG family, member 2 U72649 −1.8 −1.9 −2.4 −2.9 −1.9  
 Small inducible cytokine A3 D90144 −1.5 −1.4 −1.7 −2.4 1.5  
 Rho GDP dissociation inhibitor (GDI) X69549 −2.0 −1.9 −1.5 −1.6 −2.5 
 G-protein coupled receptor AF091890 1.3 2.6 1.2 1.3 1.3  
 Mucin 1, transmembrane X52228 2.1 1.5 1.6 1.5 1.8 
 Integrin, β 5 M35011 1.5 2.5 1.5 1.6 1.3 
 LPS-induced TNF-α factor AF010312 −3.1 −3.6 −2.8 −2.9 −2.3 
 TNF-α–induced protein 3 M59465 −2.3 −2.0 −2.8 −3.0 −2.6  
 Antigen identified by monoclonal antibody Ki-67 X65550 −2.6 −2.3 −1.2 −1.7 −2.1 
 Farnesyl diphosphate synthase D14697 −2.3 −2.2 −1.8 −1.7 −1.7 
Apoptosis/survival/drug resistance       
 Proline dehydrogenase (oxidase) 1 U82381 2.8 2.1 2.4 2.1 2.2  
 Creatine kinase X15334 2.9 1.8 1.5 1.3 1.4  
 Poly (ADP-ribose) polymerase J03473 −1.4 −1.7 −1.7 −1.6 −1.9 
 Crystalin, β B1 U35340 2.5 2.1 1.3 1.7 1.5 
 C-myc-P64 M13929 2.5 2.4 1.8 1.6 1.1 
 Casein kinase 1, epsilon L37043 −2.1 −1.7 −1.8 −1.7 −1.9 
 NM23/nonmetastatic cells 4 Y07604 −2.1 −1.9 −2.2 −2.3 −2.8 
 C-terminal binding protein 1 U37408 −2.2 −2.5 −2.4 −3.4 −2.8  
 Pyruvate kinase M26252 −3.4 −4.6 −3.1 −5.0 −6.1 
 CDC28 protein kinase 2 X54942 −1.6 −1.6 1.5 2.5 1.4  
Protein tyrosine phosphatase, nonreceptor type 6 X62055 −3.5 −3.3 −2.5 −2.8 −2.3  
 Lectin, galactoside-binding, soluble, 1 (galactin 1) AI535946 −2.5 −2.8 −3.1 −1.9 −2.3 
 V-raf-1 murine leukemia viral oncogene homolog 1 X06409 −2.5 −3.1 −2.5 −2.2 −1.8  
 Myeloid cell leukemia sequence 1 (BCL2 related) L08246 −2.2 −2.1 −2.2 −2.4 −1.9 
 Adrenomedullin D14874 −2.4 −2.6 −3.2 −4.8 −6.4 
 X-box binding protein 1 Z93930 −1.9 −2.4 −2.5 −3.2 −1.8 
 Pim-2oncogene U77735 −1.5 −1.3 −1.4 −2.3 −2.0 
Endoplasmic reticulum and translation       
 Eukaryotic translation initiation factor 5A AI074025 2.0 1.6 2.1 1.5 1.4 
 Eukaryotic translation initiation factor 4B X55733 −2.8 −2.7 −2.8 −3.1 −2.6 
 Eukaryotic translation elongation factor 1 L41498 −2.0 −2.3 −2.2 −2.1 −1.0 
 Eukaryotic translation initiation factor 3 U94855 −2.3 −2.3 −1.6 −1.5 −1.7  
 TAP binding protein (tapasin) AF029750 −2.2 −2.8 −2.6 −3.0 −1.9 
 Tumor rejection antigen (gp96) X15187 −1.9 −2.4 −2.4 −2.3 −1.4 
 Endoplasmic reticulum stress inducible AF055001 −1.5 −2.2 −2.4 −2.0 1.2 
Cytoskeleton/structural       
 Suppression of tumorigenicity 5 U15131 2.0 2.1 1.6 1.7 1.6 
 Tubulin, β, 2 X02344 −1.9 −2.4 −1.3 −1.5 −2.4 
 Actin-related protein 2/3 complex AF006088 −1.2 −1.5 −2.6 −1.3 −1.5 
 Vimentin Z19554 −2.1 −2.9 −1.7 −1.4 −1.4 
 Lymphocyte specific protein 1 M33552 −4.0 −4.2 −2.7 −2.6 −2.2  
 Adducin 1 (α) X58141 −1.7 −1.8 −2.2 −2.3 −1.9 
Ubiquitins and metabolism       
 Nucleosome assembly protein 1-like 1 W26056 −1.9 −2.5 −2.3 −2.3 −2.0 
 γ-glutamyltransferase 2 M30474 −2.0 −2.5 −1.4 −1.8 −1.3 
 Phosphomannomutase 2 U85773 2.4 1.9 1.4 1.7 1.5 
 Ubiquitin-conjugating enzyme E2M AF075599 2.2 2.1 2.6 2.4 2.0  
 Karyopherin α 2 (RAG) U28386 −1.1 −1.2 1.2 1.2 −2.1 
 Solute carrier family 25 J03592 −2.6 −2.7 −2.1 −1.9 −1.5 
 Cytochrome c oxidase subunit VIa polypeptide 1 AI540925 −1.6 −1.8 −1.3 −1.2 −1.3 
 Ubiquitin-conjugating enzyme E2G 2 AF032456 1.7 1.7 1.1 1.8 1.1 
 Ubiquitin-conjugating enzyme E2E 3 AB017644 −1.6 −1.6 −1.8 −1.6 −1.9 
 Splicing factor, arginine/serine-rich 6 AL031681 2.0 1.5 −1.0 −1.2 −1.1 
 Leukotriene A4 hydrolase J03459 −2.4 −2.4 −2.0 −2.8 −2.1 
 Ubiquitin-activating enzyme E1 M58028 −2.2 −2.8 −2.2 −2.6 −2.0  
 FK506 binding protein 1A (12 kDa) V00599 −2.1 −1.9 −1.5 −2.9 −4.5  
 RNA binding motif protein 3 U28686 1.8 2.1 1.6 1.5 1.4 

List of genes selected from Figures 1 and 2 with their potential functional categories. Fold changes shown represent the ratio of gene expression in 2ME2-treated cells versus untreated MM.1S MM cells. The gene expression values were obtained using DChip.

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