MIP-1 production by human myeloma cell lines
Cell lines . | MIP-1α (pg/106 cells/2 d) . | MIP-1β (pg/106 cells/2 d) . |
---|---|---|
ARH77 | 3.0 × 106 | 6.5 × 105 |
IM9 | 2.8 × 106 | 5.0 × 105 |
TSPC-1 | 2.9 × 106 | 8.5 × 105 |
MPC | 1.1 × 105 | 3.3 × 105 |
NPC | 6.0 × 105 | 6.0 × 105 |
OPC | 1.1 × 102 | 2.0 × 105 |
U266 | 1.0 × 106 | 1.2 × 105 |
RPMI8226 | < 5.0 | < 5.0 |
Cell lines . | MIP-1α (pg/106 cells/2 d) . | MIP-1β (pg/106 cells/2 d) . |
---|---|---|
ARH77 | 3.0 × 106 | 6.5 × 105 |
IM9 | 2.8 × 106 | 5.0 × 105 |
TSPC-1 | 2.9 × 106 | 8.5 × 105 |
MPC | 1.1 × 105 | 3.3 × 105 |
NPC | 6.0 × 105 | 6.0 × 105 |
OPC | 1.1 × 102 | 2.0 × 105 |
U266 | 1.0 × 106 | 1.2 × 105 |
RPMI8226 | < 5.0 | < 5.0 |
Myeloma cell lines were cultured at 5 × 105cells/mL for 2 days and conditioned media were harvested. MIP-1α and MIP-1β concentrations in the collected media were measured by ELISA and expressed as pg/106 cells/2 d.