Table 1.

Leukocytes recovered from peripheral organs of GVHD mice*

SpleenLiverLung
B6 female → BALB.B male    
 Day 3 after transplantation 2 × 106 3 × 105 1 × 105 
  % donor CD4 T cells 11 15  
  % donor CD8 T cells 35 52 56  
 Day 7 after transplantation 2.6 × 107 2.7 × 106 2.7 × 106 
  % donor CD4 T cells 90 99 99  
  % donor CD8 T cells 87 97 97  
 Day 14 6.4 × 107 6 × 106 4.3 × 106 
 Day 28 2.3 × 107 8.8 × 106 10.3 × 106 
BALB.B male → BALB.B male    
 Day 14 after transplantation 4.8 × 107 1.2 × 106 1.2 × 106 
SpleenLiverLung
B6 female → BALB.B male    
 Day 3 after transplantation 2 × 106 3 × 105 1 × 105 
  % donor CD4 T cells 11 15  
  % donor CD8 T cells 35 52 56  
 Day 7 after transplantation 2.6 × 107 2.7 × 106 2.7 × 106 
  % donor CD4 T cells 90 99 99  
  % donor CD8 T cells 87 97 97  
 Day 14 6.4 × 107 6 × 106 4.3 × 106 
 Day 28 2.3 × 107 8.8 × 106 10.3 × 106 
BALB.B male → BALB.B male    
 Day 14 after transplantation 4.8 × 107 1.2 × 106 1.2 × 106 
*

Means of nucleated cells from 3 mice.

Percent of donor-derived T cells in total CD4 or CD8 T cells. Donor B6 cells were distinguished from recipient BALB.B cells by allelic expression of β2-m: B6, β2-mb; BALB.B, β2-ma.

About 100% of lymphocytes were donor-derived. Data are representative of 2 experiments.

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