Table 2.

RNA expression analysis in mice receiving transplants

Geneγ/α × 100*% γ(+) RBCγ/α per γ(+) RBC
μLCR-γ 42.6 66.2 64.4 
 34.4 49.8 69.1  
 36.1 70.1 51.5  
Mean ± SD 37.7 ± 4.3 62.0 ± 10.8 61.6 ± 9.1  
HS40-11 0.6 4.8 12.5 
 1.2 2.4 50.0  
 0.8 5.6 14.3 
 1.9 13.3 14.3  
 0.7 9.4 7.4 
 4.9 23.5 20.9  
 8.2 16.7 49.1 
 5.7 43.5 13.1  
 7.6 27.1 28.0  
Mean ± SD 3.5 ± 3.1 16.3 ± 13.3 23.3 ± 16.0 
Geneγ/α × 100*% γ(+) RBCγ/α per γ(+) RBC
μLCR-γ 42.6 66.2 64.4 
 34.4 49.8 69.1  
 36.1 70.1 51.5  
Mean ± SD 37.7 ± 4.3 62.0 ± 10.8 61.6 ± 9.1  
HS40-11 0.6 4.8 12.5 
 1.2 2.4 50.0  
 0.8 5.6 14.3 
 1.9 13.3 14.3  
 0.7 9.4 7.4 
 4.9 23.5 20.9  
 8.2 16.7 49.1 
 5.7 43.5 13.1  
 7.6 27.1 28.0  
Mean ± SD 3.5 ± 3.1 16.3 ± 13.3 23.3 ± 16.0 
*

Ratio of γ-globin mRNA versus α-globin mRNA on a per-copy basis determined by RNase protection.

Percentage of RBCs expressing γ-globin determined by flow cytometry as per Figure 5B.

Control mice containing μLCR-γ transgene as per Figure5B.43 

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