Vector characterization
| Vector . | Titer . | Stability . | Expression . |
|---|---|---|---|
| HS40-5 | 2 × 106 | 12 of 12 | 203 ± 56 |
| HS40-6 | 1 × 106 | 7 of 12 | 194 ± 53* |
| HS40-9 | 3 × 106 | 11 of 12 | 82 ± 54† |
| HS40-10 | 1 × 106 | 11 of 11 | 293 ± 186 |
| HS40-11 | 3 × 105 | 8 of 8 | 545 ± 185‡ |
| Vector . | Titer . | Stability . | Expression . |
|---|---|---|---|
| HS40-5 | 2 × 106 | 12 of 12 | 203 ± 56 |
| HS40-6 | 1 × 106 | 7 of 12 | 194 ± 53* |
| HS40-9 | 3 × 106 | 11 of 12 | 82 ± 54† |
| HS40-10 | 1 × 106 | 11 of 11 | 293 ± 186 |
| HS40-11 | 3 × 105 | 8 of 8 | 545 ± 185‡ |
Titer was determined by transfer of G418 resistance to NIH3T3 cells. Stability indicates fraction of transduced MEL cell clones with intact provirus. Expression of γ-globin protein in transduced MEL clones containing intact provirus from Figure 2 was determined by flow cytometry; reported as average ± SD mfu. HS40-5 and HS40-6 were reported previously.14 15
Includes clones with intact provirus only.
P < .001 versus HS40-5 and HS40-6.
P < .01 versus HS40-10.