Table 1.

mAb and polyclonal reagents used in this study

CD/nameName/cloneConjugateSource
Lineage-depletion mAb    
 CD3 OKT3 Nil ATCC1-a 
 CD11b OKM1 Nil ATCC  
 CD14 CMRF31 Nil In house  
 CD16 HuNK-2 Nil I. McKenzie1-b 
 CD19 FMC63 Nil H. Zola1-c 
 CD56 NKH-1 Nil Beckman Coulter1-d 
 HLA-DR L243 Nil ATCC  
Phenotyping mAb    
 BDCA-3 — PE, FITC Miltenyi 
 CD1b/c* M306 Biotin Immunex1-e 
 CD1c* BDCA-1 FITC, PE Miltenyi  
 CD2 T11 PE, FITC Coulter  
 CD4 Leu-3a PE BD 
 CD7 M-T107 PE BD  
 CD11c Leu-M5 PE BD 
 CD11c 3.9 FITC Southern Biotech1-f 
 CD14 Leu-M3 PE BD  
 CD16 Leu-11a/c PE, FITC BD  
 CD20 B9E9 PE Immunotech1-g 
 CD33 P67.6 PE BD  
 CD34 Anti-HPCA-2 PE, FITC BD  
 CD40 MAB 89 PE Immunotech 
 CD52 YTH34.5 FITC Serotec1-h 
 CD56 NKH-1 PE Immunotech 
 CD64 10.1 PE Serotec 
 CD64 10.1 FITC Pharmingen1-i 
 CD80/B7-1 BBI PE Pharmingen  
 CD83 HB15a Nil, FITC, PE Immunotech  
 CD85a/ILT-5 7H5 Nil A van Agthoven1-j 
 CD85d/ILT-4 42DI Nil A van Agthoven  
 CD85j/ILT-2 HP-F1 Nil A van Agthoven 
 CD85j/ILT-2 VMP55 FITC Dako1-k 
 CD85k/ILT-3 ZM3.8 Nil A van Agthoven 
 CD86/B7-2 2331 PE Pharmingen 
 CD123 7G3 PE Pharmingen 
 CLA HECA-452 FITC Pharmingen 
 CMRF-44 — Biotin In house 
 CMRF-56 — Biotin In house 
 DEC-205/CD205 MMRI-7 Biotin In house 
 DEC-205/CD205 M335 Biotin Immunex1-j 
 DC-SIGN/CD209 AZN-D1 Nil Y. van Kooyk1-j 
 HLA-DR Immu-35T PC5 Immunotech 
 HLA-DR B-F1 FITC Serotec 
 Langerin/CD207 DCGM-4 Nil Schering-Plough1-j 
 MMR/CD206 MMR 190.BB3 Nil F. Sallusto1-j 
Detecting reagents    
 Anti-mouse Ig Polyclonal FITC, PE Silenus1-l 
 Anti-mouse Ig Polyclonal Biotin Sigma1-m 
 Streptavidin — PE, PE-Cy5 Dako 
 Avidin — FITC BD 
CD/nameName/cloneConjugateSource
Lineage-depletion mAb    
 CD3 OKT3 Nil ATCC1-a 
 CD11b OKM1 Nil ATCC  
 CD14 CMRF31 Nil In house  
 CD16 HuNK-2 Nil I. McKenzie1-b 
 CD19 FMC63 Nil H. Zola1-c 
 CD56 NKH-1 Nil Beckman Coulter1-d 
 HLA-DR L243 Nil ATCC  
Phenotyping mAb    
 BDCA-3 — PE, FITC Miltenyi 
 CD1b/c* M306 Biotin Immunex1-e 
 CD1c* BDCA-1 FITC, PE Miltenyi  
 CD2 T11 PE, FITC Coulter  
 CD4 Leu-3a PE BD 
 CD7 M-T107 PE BD  
 CD11c Leu-M5 PE BD 
 CD11c 3.9 FITC Southern Biotech1-f 
 CD14 Leu-M3 PE BD  
 CD16 Leu-11a/c PE, FITC BD  
 CD20 B9E9 PE Immunotech1-g 
 CD33 P67.6 PE BD  
 CD34 Anti-HPCA-2 PE, FITC BD  
 CD40 MAB 89 PE Immunotech 
 CD52 YTH34.5 FITC Serotec1-h 
 CD56 NKH-1 PE Immunotech 
 CD64 10.1 PE Serotec 
 CD64 10.1 FITC Pharmingen1-i 
 CD80/B7-1 BBI PE Pharmingen  
 CD83 HB15a Nil, FITC, PE Immunotech  
 CD85a/ILT-5 7H5 Nil A van Agthoven1-j 
 CD85d/ILT-4 42DI Nil A van Agthoven  
 CD85j/ILT-2 HP-F1 Nil A van Agthoven 
 CD85j/ILT-2 VMP55 FITC Dako1-k 
 CD85k/ILT-3 ZM3.8 Nil A van Agthoven 
 CD86/B7-2 2331 PE Pharmingen 
 CD123 7G3 PE Pharmingen 
 CLA HECA-452 FITC Pharmingen 
 CMRF-44 — Biotin In house 
 CMRF-56 — Biotin In house 
 DEC-205/CD205 MMRI-7 Biotin In house 
 DEC-205/CD205 M335 Biotin Immunex1-j 
 DC-SIGN/CD209 AZN-D1 Nil Y. van Kooyk1-j 
 HLA-DR Immu-35T PC5 Immunotech 
 HLA-DR B-F1 FITC Serotec 
 Langerin/CD207 DCGM-4 Nil Schering-Plough1-j 
 MMR/CD206 MMR 190.BB3 Nil F. Sallusto1-j 
Detecting reagents    
 Anti-mouse Ig Polyclonal FITC, PE Silenus1-l 
 Anti-mouse Ig Polyclonal Biotin Sigma1-m 
 Streptavidin — PE, PE-Cy5 Dako 
 Avidin — FITC BD 

ATCC indicates American Type Culture Collection; BD, Becton Dickinson.

*

CD1b/c (M306) and CD1c (BDCA-1) used interchangeably as required. Denoted as CD1b/c in text and in figures.

F1-a

Manassas, VA

F1-b

Austin Research Institute, Melbourne, Australia

F1-c

Child Health Research Institute, Adelaide, Australia

F1-d

Gladesville, Australia

F1-e

Seattle, WA

F1-f

Birmingham, AL

F1-g

Beckman Coulter, Gladesville, Australia

F1-h

Raleigh, NC

F1-i

BD Biosciences, San Diego, CA

F1-j

7th LDAW c/o Nuffield Department of Clinical Biochemistry and Cellular Science

F1-k

Botany, NSW, Australia

F1-l

Chemicon, Boronia, Victoria, Australia

F1-m

Sydney, Australia

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