Table 1. Deletion of SAG-reactive T... | American Society of Hematology

Table 1.

Deletion of SAG-reactive T cells depends on the number of adoptively transferred cells

Host	Transferred cells	SAg	SAg-reactive T cells, %^*	Control CD4⁺Vβ6⁺ T cells, %
BALB	—	—	19.5 ± 0.9	10.6 ± 0.3
BALB	—	SEB	16.6 ± 0.6	12.7 ± 0.4
SCID	BALB	—	18.6 ± 0.1	11.5 ± 0.1
SCID	10⁷BALB	SEB	26.0 ± 0.5	9.2 ± 0.4
SCID	10⁸BALB	SEB	18.4 ± 0.3	11.5 ± 0.4
SCID	2 × 10⁸BALB^‡	SEB	14.4 ± 0.5	10.3 ± 0.5
B6	—	—	3.1 ± 0.2	ND
SCID^†	10⁷B6	mtv-6	37.8 ± 6.4	ND
SCID	2 × 10⁸B6^‡	mtv-6	16.4 ± 3.9	ND

Host	Transferred cells	SAg	SAg-reactive T cells, %^*	Control CD4⁺Vβ6⁺ T cells, %
BALB	—	—	19.5 ± 0.9	10.6 ± 0.3
BALB	—	SEB	16.6 ± 0.6	12.7 ± 0.4
SCID	BALB	—	18.6 ± 0.1	11.5 ± 0.1
SCID	10⁷BALB	SEB	26.0 ± 0.5	9.2 ± 0.4
SCID	10⁸BALB	SEB	18.4 ± 0.3	11.5 ± 0.4
SCID	2 × 10⁸BALB^‡	SEB	14.4 ± 0.5	10.3 ± 0.5
B6	—	—	3.1 ± 0.2	ND
SCID^†	10⁷B6	mtv-6	37.8 ± 6.4	ND
SCID	2 × 10⁸B6^‡	mtv-6	16.4 ± 3.9	ND

Varying numbers of allogeneic (B6) or syngeneic (BALB) cells (a mixture of spleen cells and PLNCs) were injected into SCID mice. Allogeneic T cells were stimulated by the mtv-6 SAg during GVHD and syngeneic T cells were stimulated by concomitantly injected SEB. Twelve days later, the percentage of SAg-reactive cells (CD4⁺Vβ3⁺ T cells in GVHD and CD4⁺Vβ8⁺ T cells for SEB) in the lymph nodes of host mice were determined by flow cytometry. The percentage of CD4⁺Vβ6⁺ T cells, which should not be stimulated by superantigens, was also determined as a control. The data are pooled from 4 different experiments and the average and SE is reported. (n = 2, at least, for each experiment involving adoptive transfer).

ND indicates not done.

CD4⁺Vβ8⁺ T cells in the case of SEB and CD4⁺Vβ3⁺ T cells in the case ofmtv-6.

†

For GVHD studies, SCID mice were sublethally irradiated (275 cGy).

‡

1 × 10⁸ cells were injected twice, 24 hours apart.

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