B-cell subset frequencies in IUT, TBI, and control mice
| Mouse group* . | Percent of total B cells ± SD . | ||||||
|---|---|---|---|---|---|---|---|
| BM early . | BM mature . | Spleen follicular . | Spleen MZ . | Spleen immature . | PC B1 . | PC B2 . | |
| C57B1/6 control | 71.8 ± 14.3 | 28.2 ± 14.3 | 68.5 ± 8.4 | 23.4 ± 11.0 | 8.1 ± 4.4 | 65.5 ± 20.1 | 34.5 ± 20.1 |
| BALB/c control | 68.8 ± 8.1 | 31.2 ± 8.1 | 80.7 ± 3.0 | 16.2 ± 3.7 | 3.2 ± 1.0 | 55.3 ± 13.2 | 44.7 ± 13.2 |
| Congenic IUT | 58.5 ± 16.7 | 41.5 ± 16.7 | 69.5 ± 7.3 | 26.3 ± 8.5 | 4.2 ± 1.7 | 59.6 ± 16.2 | 40.4 ± 16.2 |
| Allo NTCD IUT | 58.6 ± 13.6 | 41.4 ± 13.6 | 65.4 ± 9.1 | 26.9 ± 11.5 | 7.6 ± 2.8 | 54.8 ± 20.2 | 45.2 ± 20.2 |
| Allo TCD IUT | 63.9 ± 14.3 | 36.1 ± 14.3 | 57.8 ± 10.5 | 36.1 ± 8.3 | 6.1 ± 4.9 | 56.5 ± 18.0 | 43.5 ± 18.0 |
| Allo TBI | 58.5 ± 14.3 | 41.5 ± 14.3 | 79.7 ± 6.8 | 10.5 ± 2.9 | 9.8 ± 6.4 | 38.2 ± 18.3 | 61.8 ± 18.3 |
| Mouse group* . | Percent of total B cells ± SD . | ||||||
|---|---|---|---|---|---|---|---|
| BM early . | BM mature . | Spleen follicular . | Spleen MZ . | Spleen immature . | PC B1 . | PC B2 . | |
| C57B1/6 control | 71.8 ± 14.3 | 28.2 ± 14.3 | 68.5 ± 8.4 | 23.4 ± 11.0 | 8.1 ± 4.4 | 65.5 ± 20.1 | 34.5 ± 20.1 |
| BALB/c control | 68.8 ± 8.1 | 31.2 ± 8.1 | 80.7 ± 3.0 | 16.2 ± 3.7 | 3.2 ± 1.0 | 55.3 ± 13.2 | 44.7 ± 13.2 |
| Congenic IUT | 58.5 ± 16.7 | 41.5 ± 16.7 | 69.5 ± 7.3 | 26.3 ± 8.5 | 4.2 ± 1.7 | 59.6 ± 16.2 | 40.4 ± 16.2 |
| Allo NTCD IUT | 58.6 ± 13.6 | 41.4 ± 13.6 | 65.4 ± 9.1 | 26.9 ± 11.5 | 7.6 ± 2.8 | 54.8 ± 20.2 | 45.2 ± 20.2 |
| Allo TCD IUT | 63.9 ± 14.3 | 36.1 ± 14.3 | 57.8 ± 10.5 | 36.1 ± 8.3 | 6.1 ± 4.9 | 56.5 ± 18.0 | 43.5 ± 18.0 |
| Allo TBI | 58.5 ± 14.3 | 41.5 ± 14.3 | 79.7 ± 6.8 | 10.5 ± 2.9 | 9.8 ± 6.4 | 38.2 ± 18.3 | 61.8 ± 18.3 |
BM and spleen B-cell percentages are presented as a proportion of total B220+ cells, and PC B-cell percentages as a proportion of total IgM+ cells. Values are mean ± SD. IUT mice were examined starting at 12 weeks of age. TBI mice were tested starting at 12 weeks after transplantation. B-cell subsets were defined as follows: BM early indicates B220+CD23−; BM mature, B220+CD23+; spleen follicular, B220+CD21/35intHSAlow; spleen MZ, B220+CD21/35highHSAint; spleen immature, B220+CD21/35lowHSAhigh; PC B1, IgM+CD23−; and PC B2, IgM+CD23+.
n values for each group range from 6 to 11.