Total B-cell recovery in IUT, TBI, and control mice
| Mouse group* . | Total BM B cells (× 106) . | BM B-cell frequency, % . | Total splenic B cells (× 106) . | Splenic B-cell frequency, % . |
|---|---|---|---|---|
| C57B1/6 control | 3.2 ± 0.9 | 21.1 ± 5.0 | 47.6 ± 18.3 | 56.0 ± 9.7 |
| BALB/c control | 2.1 ± 0.8 | 16.2 ± 3.7 | 54.4 ± 9.2 | 57.6 ± 3.5 |
| Congenic IUT | 1.1 ± 0.6 | 11.6 ± 4.4 | 37.6 ± 19.9 | 44.2 ± 7.2 |
| Allo NTCD IUT | 3.7 ± 2.6 | 16.9 ± 5.2 | 45.4 ± 10.5 | 55.9 ± 6.8 |
| Allo TCD IUT | 1.6 ± 1.7 | 13.8 ± 13.1 | 25.8 ± 9.1 | 43.6 ± 9.1 |
| Allo TBI | 3.3 ± 2.2 | 19.3 ± 4.4 | 38.3 ± 10.3 | 58.8 ± 3.3 |
| Mouse group* . | Total BM B cells (× 106) . | BM B-cell frequency, % . | Total splenic B cells (× 106) . | Splenic B-cell frequency, % . |
|---|---|---|---|---|
| C57B1/6 control | 3.2 ± 0.9 | 21.1 ± 5.0 | 47.6 ± 18.3 | 56.0 ± 9.7 |
| BALB/c control | 2.1 ± 0.8 | 16.2 ± 3.7 | 54.4 ± 9.2 | 57.6 ± 3.5 |
| Congenic IUT | 1.1 ± 0.6 | 11.6 ± 4.4 | 37.6 ± 19.9 | 44.2 ± 7.2 |
| Allo NTCD IUT | 3.7 ± 2.6 | 16.9 ± 5.2 | 45.4 ± 10.5 | 55.9 ± 6.8 |
| Allo TCD IUT | 1.6 ± 1.7 | 13.8 ± 13.1 | 25.8 ± 9.1 | 43.6 ± 9.1 |
| Allo TBI | 3.3 ± 2.2 | 19.3 ± 4.4 | 38.3 ± 10.3 | 58.8 ± 3.3 |
BM and spleen cells were harvested and spun over FicoLite-LM to isolate viable mononuclear cells. After cell counts, cell suspensions were stained with anti-B220 mAb, followed by flow cytometric analysis. Percentage of B220+ cells was determined by software gating. Values are mean ± SD. IUT mice were examined starting at 12 weeks of age; TBI mice were tested starting at 12 weeks after transplantation. Allo NTCD indicates recipients of allogeneic non-TCD BM; allo TCD, recipients of allogeneic TCD BM.
n values for each group range from 6 to 11.