Time course of epitope specificity (tetramer staining) and phenotype of mini-LCL–stimulated T-cell cultures from donor no. 5
| . | pp65 mini-LCL–stimulated T cells . | Control mini-LCL–stimulated T cells . | ||||||
|---|---|---|---|---|---|---|---|---|
| No. of restimulations | 2 | 3 | 4 | 6 | 2 | 3 | 4 | 6 |
| Day of restimulation | 8 | 15 | 22 | 40 | 8 | 15 | 22 | 40 |
| Total cell number (million) | 9 | 58 | 184 | 645 | 7 | 44 | 78 | 87 |
| Day of analysis | 21 | 26 | 29 | 49 | 21 | 26 | 29 | 49 |
| % NLV (CMV pp65) | 3.01 | 6.37 | 10.77 | 15.26 | 0.01 | 0.00 | 0.00 | 0.00 |
| % CLG (EBV LMP2) | 0.30 | 0.25 | 0.14 | 0.07 | 1.00 | 1.01 | 0.76 | 2.50 |
| % CD8+CD3+ | 34.2 | 72.4 | 79.6 | 78.1 | 28.1 | 48.3 | 53.4 | 53.3 |
| % CD4+CD3+ | 13.8 | 11.9 | 13.6 | 19.7 | 6.6 | 7.7 | 14.3 | 20.5 |
| % CD56+CD3− | 35.3 | 16.4 | 3.7 | 0.25 | 53.0 | 41.5 | 20.0 | 14.2 |
| . | pp65 mini-LCL–stimulated T cells . | Control mini-LCL–stimulated T cells . | ||||||
|---|---|---|---|---|---|---|---|---|
| No. of restimulations | 2 | 3 | 4 | 6 | 2 | 3 | 4 | 6 |
| Day of restimulation | 8 | 15 | 22 | 40 | 8 | 15 | 22 | 40 |
| Total cell number (million) | 9 | 58 | 184 | 645 | 7 | 44 | 78 | 87 |
| Day of analysis | 21 | 26 | 29 | 49 | 21 | 26 | 29 | 49 |
| % NLV (CMV pp65) | 3.01 | 6.37 | 10.77 | 15.26 | 0.01 | 0.00 | 0.00 | 0.00 |
| % CLG (EBV LMP2) | 0.30 | 0.25 | 0.14 | 0.07 | 1.00 | 1.01 | 0.76 | 2.50 |
| % CD8+CD3+ | 34.2 | 72.4 | 79.6 | 78.1 | 28.1 | 48.3 | 53.4 | 53.3 |
| % CD4+CD3+ | 13.8 | 11.9 | 13.6 | 19.7 | 6.6 | 7.7 | 14.3 | 20.5 |
| % CD56+CD3− | 35.3 | 16.4 | 3.7 | 0.25 | 53.0 | 41.5 | 20.0 | 14.2 |
To determine the total cell number, trypan blue–excluding cells were visually identified and counted on the day of restimulation preceding each analysis. Each T-cell culture was initiated with 3 million PBMC (day 0). For tetramer and phenotypic analyses, percentages of total cells are given. .