Fold increase in survivin and Ki-67 in G0CD34+ cells after growth factor addition
| . | 0 hours after growth factor addition3-150 . | 12 hours after growth factor addition3-150 . |
|---|---|---|
| Fold increase survivin protein | – | 2.1 ± 0.33-151(1.9 ± 0.1)3-151 |
| % survivin-positive cells | 4.7 ± 1.4 (1.6 ± 0.7) | 32.5 ± 9.33-151(40.0 ± 18.5)3-151 |
| Fold increase survivin mRNA | – | 2.4 ± 0.73-152 |
| Fold increase Ki-67 protein | – | 0.9 ± 0.1 |
| % Ki-67negativecells | 89.9 ± 9.1 | 95.2 ± 4.3 |
| Fold increase Ki-67 mRNA | – | 0.8 ± 0.3 |
| . | 0 hours after growth factor addition3-150 . | 12 hours after growth factor addition3-150 . |
|---|---|---|
| Fold increase survivin protein | – | 2.1 ± 0.33-151(1.9 ± 0.1)3-151 |
| % survivin-positive cells | 4.7 ± 1.4 (1.6 ± 0.7) | 32.5 ± 9.33-151(40.0 ± 18.5)3-151 |
| Fold increase survivin mRNA | – | 2.4 ± 0.73-152 |
| Fold increase Ki-67 protein | – | 0.9 ± 0.1 |
| % Ki-67negativecells | 89.9 ± 9.1 | 95.2 ± 4.3 |
| Fold increase Ki-67 mRNA | – | 0.8 ± 0.3 |
Survivin and Ki-67 protein expression (means ± SEM from 3 experiments) was determined by intracellular flow cytometry; mRNA levels were determined by real-time RT-PCR (means ± SD from 2 experiments). Data are expressed as the fold increase in survivin and Ki-67 protein or percentage survivin-positive or Ki-67negative cells in G0 CD34+cells or in the G0 CD34+Ki-67negative cell population (shown in parentheses).
100 ng/mL each rhTpo, rhFL, and rhSCF.
P < .005.
Range, 1.42-3.27-fold; n = 2 experiments.