Table 2. Survivin expression in CD34... | American Society of Hematology

Table 2.

Survivin expression in CD34⁺ cell populations following growth factor addition

Cell population	Fold increase in survivin protein
Cell population	2 hours after growth factor addition^*	4 hours after growth factor addition^*	6 hours after growth factor addition^*
CD34⁺, cyclin D^negative	1.8 ± 0.3^†	2.2 ± 0.4^‡	2.4 ± 0.4^‡
CD34⁺, cyclin D^positive	1.7 ± 0.2^‡	2.3 ± 0.5^‡	2.7 ± 0.5^‡
CD34⁺, Ki-67^negative	2.4 ± 0.2^2-153	3.2 ± 0.2^2-153	4.2 ± 0.5^2-153
CD34⁺, Ki-67^positive	3.9 ± 1.0^‡	5.4 ± 1.8^†	6.5 ± 2.0^‡
CD34⁺, underphosphorylated Rb^positive	2.2 ± 0.2^2-153	3.2 ± 0.3^2-153	3.7 ± 0.6^2-153
CD34⁺, underphosphorylated Rb^negative	1.9 ± 0.3^‡	3.2 ± 0.5^2-153	3.5 ± 0.6^2-153

Cell population	Fold increase in survivin protein
Cell population	2 hours after growth factor addition^*	4 hours after growth factor addition^*	6 hours after growth factor addition^*
CD34⁺, cyclin D^negative	1.8 ± 0.3^†	2.2 ± 0.4^‡	2.4 ± 0.4^‡
CD34⁺, cyclin D^positive	1.7 ± 0.2^‡	2.3 ± 0.5^‡	2.7 ± 0.5^‡
CD34⁺, Ki-67^negative	2.4 ± 0.2^2-153	3.2 ± 0.2^2-153	4.2 ± 0.5^2-153
CD34⁺, Ki-67^positive	3.9 ± 1.0^‡	5.4 ± 1.8^†	6.5 ± 2.0^‡
CD34⁺, underphosphorylated Rb^positive	2.2 ± 0.2^2-153	3.2 ± 0.3^2-153	3.7 ± 0.6^2-153
CD34⁺, underphosphorylated Rb^negative	1.9 ± 0.3^‡	3.2 ± 0.5^2-153	3.5 ± 0.6^2-153

Survivin, cyclin D, Ki-67, and underphosphorylated-Rb protein levels were determined by intracellular flow cytometry in the CD34⁺ cell population from UCB. Data are expressed as means ± SEM from 3 experiments.

100 ng/mL each rhTpo, rhFL, rhSCF.

†

P < .05.

‡

P < .01.

F2-153

P < .005 compared with time 0.

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