Table 1. Survivin, Ki-67, cyclin D,... | American Society of Hematology

Table 1.

Survivin, Ki-67, cyclin D, phosphorylated-Rb, underphosphorylated-Rb, and % S + G₂/M cells in Tpo-, SCF-, and FL-stimulated CD34⁺ cells

Cell population	No. of experiments	Fold increase
Cell population	No. of experiments	2 hours after growth factor addition^*	4 hours after growth factor addition^*	6 hours after growth factor addition^*	18 hours after growth factor addition^*
Survivin protein	4	1.4 ± 0.2	1.8 ± 0.2^‡	2.4 ± 0.5^†	6.2 ± 2.4^†
Survivin mRNA	3	1.1 ± 0.1	1.2 ± 0.1^†	2.1 ± 0.5^†	10.3 ± 3.8^†
%S + G₂M phase	4	7.5 ± 1.2	6.9 ± 1.0	8.5 ± 1.2^†	19.9 ± 3.1^†
Ki-67	2	1.3 ± 0.01^1-153	1.3 ± 0.02^1-153	1.2 ± 0.01^1-153	1.5 ± 0.2^1-153
Cyclin D	5	1.1 ± 0.1^†	1.2 ± 0.1^†	1.2 ± 0.1^‡	1.5 ± 0.2^†
Phosphorylated Rb/Total Rb	2	1.7 ± 0.6^†	1.5 ± 0.5^†	1.8 ± 0.7^†	2.3 ± 0.6^†
Underphosphorylated Rb/Total Rb	3	0.8 ± 0.1^†	0.7 ± 0.1^‡	0.7 ± 0.1^†	0.5 ± 0.1^1-153

Cell population	No. of experiments	Fold increase
Cell population	No. of experiments	2 hours after growth factor addition^*	4 hours after growth factor addition^*	6 hours after growth factor addition^*	18 hours after growth factor addition^*
Survivin protein	4	1.4 ± 0.2	1.8 ± 0.2^‡	2.4 ± 0.5^†	6.2 ± 2.4^†
Survivin mRNA	3	1.1 ± 0.1	1.2 ± 0.1^†	2.1 ± 0.5^†	10.3 ± 3.8^†
%S + G₂M phase	4	7.5 ± 1.2	6.9 ± 1.0	8.5 ± 1.2^†	19.9 ± 3.1^†
Ki-67	2	1.3 ± 0.01^1-153	1.3 ± 0.02^1-153	1.2 ± 0.01^1-153	1.5 ± 0.2^1-153
Cyclin D	5	1.1 ± 0.1^†	1.2 ± 0.1^†	1.2 ± 0.1^‡	1.5 ± 0.2^†
Phosphorylated Rb/Total Rb	2	1.7 ± 0.6^†	1.5 ± 0.5^†	1.8 ± 0.7^†	2.3 ± 0.6^†
Underphosphorylated Rb/Total Rb	3	0.8 ± 0.1^†	0.7 ± 0.1^‡	0.7 ± 0.1^†	0.5 ± 0.1^1-153

Survivin, cyclin D, Ki-67, phosphorylated-Rb (Ser 807/811), underphosphorylated-Rb, and total-Rb protein levels were determined by intracellular flow cytometry in the CD34⁺ cell population from UCB. The fold increases in mean channel fluorescence for each protein and the ratio of fold changes of phosphorylated Rb/total Rb and underphosphorylated Rb/total Rb are shown as means ± SEM of the number of experiments indicated. Survivin mRNA was determined by real-time RT-PCR. The percentage of CD34⁺ cells in S + G₂/M phase was measured by DNA staining with 7-AAD and analyzed using ModFit software. Data are presented as means ± SEM of 4 experiments.

100 ng/mL each rhTpo, rhFL, rhSCF.

†

P < .05.

‡

P < .01.

F1-153

P < .005 compared with time 0.

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