Analysis of DC-associated gene expression at different maturation stages for each DC subset
| Gene no. . | Name . | Ratio of DC and BMT . | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Mo DCs . | CD1a+DCs . | CD11c−DCs . | CD14+DCs . | ||||||
| Day 7 . | Day 9 . | Day 8 . | Day 18 . | Day 0 . | Day 5 . | Day 8 . | Day 18 . | ||
| 2 | S100B | 1.4 | 0.9 | 1.9 | 28.6 | 0.7 | 0.9 | 0.9 | 5.6 |
| 3 | MMP12 | 0.6 | 0.7 | 0.4 | 4.9 | 0.5 | 0.7 | 0.5 | 0.3 |
| 4 | TARC | 2.0 | 20.0 | 0.7 | 24.0 | 0.6 | 0.9 | 0.7 | 0.8 |
| 5 | MYPT1 | 0.6 | 1.0 | 0.6 | 2.0 | 0.4 | 0.4 | 1.0 | 4.9 |
| 6 | CD1B | 1.5 | 2.4 | 1.4 | 17.6 | 0.8 | 0.9 | 0.7 | 1.5 |
| 7 | CD20-like | 1.9 | 0.8 | 6.3 | 18.0 | 1.7 | 0.6 | 6.2 | 20.7 |
| 8 | Ig superfamily Z39Ig | 0.4 | 0.9 | 0.3 | 2.9 | 0.6 | 0.8 | 0.5 | 16.4 |
| 9 | HLA-DQα | 1.2 | 3.2 | 0.8 | 9.6 | 0.5 | 0.5 | 1.0 | 2.0 |
| 10 | GPNMB | 0.4 | 1.7 | 0.7 | 1.2 | 0.8 | 0.7 | 1.0 | 5.0 |
| 11 | Eta-1 | 0.8 | 3.2 | 0.4 | 6.3 | 0.5 | 0.5 | 0.7 | 11.8 |
| 12 | FLAP | 2.7 | 0.9 | 6.8 | 8.1 | 3.0 | 4.9 | 7.0 | 5.4 |
| 13 | MRC1 | 0.9 | 1.6 | 2.1 | 3.8 | 0.7 | 1.0 | 1.2 | 6.1 |
| 15 | MCP4 | 1.1 | 2.9 | 0.3 | 4.5 | 0.4 | 0.6 | 1.2 | 5.6 |
| 16 | HLA-DRα | 2.6 | 2.2 | 3.5 | 3.8 | 2.4 | 3.3 | 3.9 | 1.3 |
| 17 | HLA-DRβ | 1.4 | 0.7 | 1.8 | 2.7 | 1.2 | 0.7 | 1.2 | 1.4 |
| 18 | RNase1 | 0.3 | 1.6 | 0.3 | 1.1 | 0.4 | 0.5 | 0.5 | 11.5 |
| 19 | DRP-2 | 0.9 | 8.9 | 6.4 | 2.4 | 5.9 | 5.7 | 7.9 | 1.1 |
| 22 | Lysosomal acid lipase | 3.4 | 4.2 | 0.3 | 8.1 | 0.3 | 0.4 | 0.5 | 6.1 |
| 23 | Cystatin A | 0.5 | 0.8 | 0.3 | 17.4 | 0.3 | 0.4 | 0.4 | 4.3 |
| 24 | CD36 | 0.4 | 0.8 | 0.8 | 2.9 | 0.8 | 0.8 | 0.9 | 1.2 |
| 26 | MCP1 | 0.3 | 0.7 | 0.2 | 0.5 | 0.2 | 0.3 | 3.3 | 9.6 |
| 28 | TGFBI | 1.8 | 1.8 | 0.5 | 3.4 | 1.5 | 0.9 | 0.7 | 9.2 |
| 30 | Calmodulin | 0.8 | 5.1 | 0.5 | 1.8 | 0.4 | 0.7 | 0.6 | 1.6 |
| 31 | MDL-1 | 0.4 | 1.0 | 0.4 | 1.3 | 0.6 | 0.8 | 0.7 | 1.9 |
| 33 | β-tubulin | 1.1 | 2.8 | 0.4 | 2.8 | 0.3 | 0.3 | 0.6 | 2.7 |
| 37 | Enolase 1 | 0.6 | 3.8 | 0.3 | 1.4 | 0.1 | 0.1 | 0.4 | 1.5 |
| 40 | Thymosin β4 | 1.2 | 1.9 | 0.3 | 3.4 | 0.1 | 0.3 | 0.6 | 1.6 |
| 41 | Ferritin | 1.3 | 3.7 | 0.1 | 1.9 | 0.0 | 0.1 | 0.3 | 5.1 |
| 44 | Annexin A2 | 0.4 | 3.1 | 0.1 | 4.8 | 0.1 | 0.1 | 0.3 | 2.2 |
| 46 | MGST2 | 0.6 | 1.0 | 0.6 | 3.5 | 0.5 | 0.4 | 1.1 | 3.5 |
| 49 | Factor XIIIa | 0.3 | 1.0 | 0.4 | 0.7 | 0.5 | 0.7 | 0.8 | 3.0 |
| 52 | VAMP8 | 2.0 | 2.8 | 0.6 | 5.1 | 0.3 | 0.2 | 1.3 | 4.2 |
| 54 | Purinergic receptor | 0.4 | 1.0 | 0.6 | 3.1 | 0.6 | 0.6 | 1.0 | 1.9 |
| 55 | ARHGDIB | 0.1 | 0.2 | 0.1 | 1.0 | 0.1 | 0.1 | 0.3 | 2.0 |
| 58 | MHC-DMα | 0.8 | 1.9 | 1.3 | 4.1 | 0.6 | 0.4 | 2.2 | 2.8 |
| 61 | DORA | 1.7 | 0.9 | 0.5 | 4.0 | 0.4 | 0.6 | 0.7 | 1.8 |
| 63 | IRF4 | 0.3 | 7.9 | 10.0 | 2.2 | 9.3 | 0.9 | 5.8 | 0.4 |
| S4 | DC-Lamp | 0.3 | 7.4 | 1.4 | 6.3 | 0.4 | 0.8 | 0.5 | 0.4 |
| S5 | E-cadherin | 0.5 | 1.0 | 0.6 | 1.0 | 0.9 | 0.9 | 0.5 | 0.9 |
| S6 | DEC205 (CD205) | 0.4 | 2.9 | 0.3 | 1.6 | 0.8 | 0.6 | 0.5 | 0.7 |
| S7 | Mannose receptor (CD206) | 1.1 | 1.8 | 3.1 | 6.6 | 0.4 | 0.5 | 2.0 | 8.6 |
| S8 | Langerin (CD207) | 0.4 | 1.2 | 0.5 | 5.9 | 0.8 | 0.7 | 0.6 | 1.0 |
| Gene no. . | Name . | Ratio of DC and BMT . | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Mo DCs . | CD1a+DCs . | CD11c−DCs . | CD14+DCs . | ||||||
| Day 7 . | Day 9 . | Day 8 . | Day 18 . | Day 0 . | Day 5 . | Day 8 . | Day 18 . | ||
| 2 | S100B | 1.4 | 0.9 | 1.9 | 28.6 | 0.7 | 0.9 | 0.9 | 5.6 |
| 3 | MMP12 | 0.6 | 0.7 | 0.4 | 4.9 | 0.5 | 0.7 | 0.5 | 0.3 |
| 4 | TARC | 2.0 | 20.0 | 0.7 | 24.0 | 0.6 | 0.9 | 0.7 | 0.8 |
| 5 | MYPT1 | 0.6 | 1.0 | 0.6 | 2.0 | 0.4 | 0.4 | 1.0 | 4.9 |
| 6 | CD1B | 1.5 | 2.4 | 1.4 | 17.6 | 0.8 | 0.9 | 0.7 | 1.5 |
| 7 | CD20-like | 1.9 | 0.8 | 6.3 | 18.0 | 1.7 | 0.6 | 6.2 | 20.7 |
| 8 | Ig superfamily Z39Ig | 0.4 | 0.9 | 0.3 | 2.9 | 0.6 | 0.8 | 0.5 | 16.4 |
| 9 | HLA-DQα | 1.2 | 3.2 | 0.8 | 9.6 | 0.5 | 0.5 | 1.0 | 2.0 |
| 10 | GPNMB | 0.4 | 1.7 | 0.7 | 1.2 | 0.8 | 0.7 | 1.0 | 5.0 |
| 11 | Eta-1 | 0.8 | 3.2 | 0.4 | 6.3 | 0.5 | 0.5 | 0.7 | 11.8 |
| 12 | FLAP | 2.7 | 0.9 | 6.8 | 8.1 | 3.0 | 4.9 | 7.0 | 5.4 |
| 13 | MRC1 | 0.9 | 1.6 | 2.1 | 3.8 | 0.7 | 1.0 | 1.2 | 6.1 |
| 15 | MCP4 | 1.1 | 2.9 | 0.3 | 4.5 | 0.4 | 0.6 | 1.2 | 5.6 |
| 16 | HLA-DRα | 2.6 | 2.2 | 3.5 | 3.8 | 2.4 | 3.3 | 3.9 | 1.3 |
| 17 | HLA-DRβ | 1.4 | 0.7 | 1.8 | 2.7 | 1.2 | 0.7 | 1.2 | 1.4 |
| 18 | RNase1 | 0.3 | 1.6 | 0.3 | 1.1 | 0.4 | 0.5 | 0.5 | 11.5 |
| 19 | DRP-2 | 0.9 | 8.9 | 6.4 | 2.4 | 5.9 | 5.7 | 7.9 | 1.1 |
| 22 | Lysosomal acid lipase | 3.4 | 4.2 | 0.3 | 8.1 | 0.3 | 0.4 | 0.5 | 6.1 |
| 23 | Cystatin A | 0.5 | 0.8 | 0.3 | 17.4 | 0.3 | 0.4 | 0.4 | 4.3 |
| 24 | CD36 | 0.4 | 0.8 | 0.8 | 2.9 | 0.8 | 0.8 | 0.9 | 1.2 |
| 26 | MCP1 | 0.3 | 0.7 | 0.2 | 0.5 | 0.2 | 0.3 | 3.3 | 9.6 |
| 28 | TGFBI | 1.8 | 1.8 | 0.5 | 3.4 | 1.5 | 0.9 | 0.7 | 9.2 |
| 30 | Calmodulin | 0.8 | 5.1 | 0.5 | 1.8 | 0.4 | 0.7 | 0.6 | 1.6 |
| 31 | MDL-1 | 0.4 | 1.0 | 0.4 | 1.3 | 0.6 | 0.8 | 0.7 | 1.9 |
| 33 | β-tubulin | 1.1 | 2.8 | 0.4 | 2.8 | 0.3 | 0.3 | 0.6 | 2.7 |
| 37 | Enolase 1 | 0.6 | 3.8 | 0.3 | 1.4 | 0.1 | 0.1 | 0.4 | 1.5 |
| 40 | Thymosin β4 | 1.2 | 1.9 | 0.3 | 3.4 | 0.1 | 0.3 | 0.6 | 1.6 |
| 41 | Ferritin | 1.3 | 3.7 | 0.1 | 1.9 | 0.0 | 0.1 | 0.3 | 5.1 |
| 44 | Annexin A2 | 0.4 | 3.1 | 0.1 | 4.8 | 0.1 | 0.1 | 0.3 | 2.2 |
| 46 | MGST2 | 0.6 | 1.0 | 0.6 | 3.5 | 0.5 | 0.4 | 1.1 | 3.5 |
| 49 | Factor XIIIa | 0.3 | 1.0 | 0.4 | 0.7 | 0.5 | 0.7 | 0.8 | 3.0 |
| 52 | VAMP8 | 2.0 | 2.8 | 0.6 | 5.1 | 0.3 | 0.2 | 1.3 | 4.2 |
| 54 | Purinergic receptor | 0.4 | 1.0 | 0.6 | 3.1 | 0.6 | 0.6 | 1.0 | 1.9 |
| 55 | ARHGDIB | 0.1 | 0.2 | 0.1 | 1.0 | 0.1 | 0.1 | 0.3 | 2.0 |
| 58 | MHC-DMα | 0.8 | 1.9 | 1.3 | 4.1 | 0.6 | 0.4 | 2.2 | 2.8 |
| 61 | DORA | 1.7 | 0.9 | 0.5 | 4.0 | 0.4 | 0.6 | 0.7 | 1.8 |
| 63 | IRF4 | 0.3 | 7.9 | 10.0 | 2.2 | 9.3 | 0.9 | 5.8 | 0.4 |
| S4 | DC-Lamp | 0.3 | 7.4 | 1.4 | 6.3 | 0.4 | 0.8 | 0.5 | 0.4 |
| S5 | E-cadherin | 0.5 | 1.0 | 0.6 | 1.0 | 0.9 | 0.9 | 0.5 | 0.9 |
| S6 | DEC205 (CD205) | 0.4 | 2.9 | 0.3 | 1.6 | 0.8 | 0.6 | 0.5 | 0.7 |
| S7 | Mannose receptor (CD206) | 1.1 | 1.8 | 3.1 | 6.6 | 0.4 | 0.5 | 2.0 | 8.6 |
| S8 | Langerin (CD207) | 0.4 | 1.2 | 0.5 | 5.9 | 0.8 | 0.7 | 0.6 | 1.0 |
DC-associated genes listed in Table 1 were further analyzed at different maturation stages for each DC subset using an HI380 microarray (Creagene). Genes are listed in abbreviated form according to the number used in Tables 1 and 2. The microarray was screened with probes prepared from each DC subset (purity more than 98%) at different maturation stages and from BMTs without subtraction. The ratio of DCs to BMTs for each gene was determined from 2 different sets of experiments after background subtraction and normalization. Genes showing little difference between their mature and immature stages were omitted from the list shown in Table 2.