DC-associated genes identified from subtraction, microarray, and sequence analysis
| Gene no. . | Name . | GenBank accession no. . | Redundancy in screening . | Intensity of BMT . | Intensity of DCs . | DC/BMT . |
|---|---|---|---|---|---|---|
| 1 | α-tubulin* | K00558 | 4 | 1 312 | 37 669 | 28.7 |
| 2 | S100 calcium-binding protein, β (S100B) | NM_006272 | 2 | 1 036 | 28 546 | 27.6 |
| 3 | Matrix metalloproteinase-12 (MMP12)* | XM_006272 | 5 | 1 402 | 38 171 | 27.2 |
| 4 | Thymus and activation-regulated chemokine (TARC) | D43767 | 1 | 1 109 | 26 047 | 23.5 |
| 5 | Myosin phosphatase, target subunit 1 (MYPT1) | XM_006578 | 1 | 2 376 | 43 787 | 18.4 |
| 6 | CD1B antigen (CD1B) | XM_002174 | 4 | 1 286 | 20 646 | 16.1 |
| 7 | CD20-like precursor | NM_022349 | 15 | 1 746 | 27 393 | 15.7 |
| 8 | Ig superfamily protein (Z39Ig)* | XM_010265 | 23 | 1 430 | 18 815 | 13.2 |
| 9 | MHC class II HLA-DQα chain | U77589 | 1 | 1 071 | 13 284 | 12.4 |
| 10 | Glycoprotein NMB (GPNMB) | XM_004781 | 3 | 1 170 | 13 914 | 11.9 |
| 11 | Osteopontin* | NM_000582 | 6 | 2 964 | 33 178 | 11.2 |
| 12 | 5-lipoxygenase activating protein (FLAP) | M63262 | 6 | 1 126 | 12 316 | 10.9 |
| 13 | Mannose receptor C, type 1 (MRC1) | NM_002438 | 3 | 1 284 | 13 960 | 10.9 |
| 14 | Cytochrome b-245, β polypeptide (CYBB) | NM_000397 | 3 | 1 050 | 11 226 | 10.7 |
| 15 | Monocyte chemotactic proteins 4 (MCP4; SCYA13) | XM_008411 | 4 | 1 429 | 14 456 | 10.1 |
| 16 | MHC class II HLA-DRα chain* | XM_004209 | 8 | 1 454 | 14 578 | 10.0 |
| 17 | MHC class II HLA-DRβ chain | M26038 | 6 | 1 607 | 14 838 | 9.2 |
| 18 | RNase A family 1 (RNASE1) | XM_012375 | 2 | 2 413 | 21 588 | 8.9 |
| 19 | Dihydropyrimidinase related protein-2 | D78013 | 4 | 1 997 | 17 249 | 8.6 |
| 20 | ATP synthase 6 (MTATP6; mitochondria) | AF347015 | 11 | 3 394 | 26 657 | 7.9 |
| 21 | 16S ribosomal RNA (mitochondria)* | AF347015 | 6 | 2 683 | 21 015 | 7.8 |
| 22 | Lysosomal acid lipase | U08464 | 22 | 1 043 | 7 756 | 7.4 |
| 23 | Cystatin A (CSTA) | NM_005213 | 4 | 1 286 | 9 437 | 7.3 |
| 24 | Antigen CD36 | M98399 | 8 | 1 404 | 9 501 | 6.8 |
| 25 | Immunoglobulin transcription factor-2 (IFT2) | XM_012756 | 1 | 1 719 | 11 613 | 6.8 |
| 26 | Monocyte chemotactic proteins-1 (MCP1; MCAF) | M24545 | 2 | 2 311 | 14 802 | 6.4 |
| 27 | β-actin | BC004251 | 1 | 715 | 4 561 | 6.4 |
| 28 | Transforming growth factor, β-induced, 68 kD (TGFBI) | NM_000358 | 2 | 1 318 | 8 002 | 6.1 |
| 29 | Cytochrome b (MTCYB; mitochondria)* | AF347015 | 2 | 3 660 | 21 907 | 6.0 |
| 30 | Calmodulin | D45887 | 3 | 752 | 4 383 | 5.8 |
| 31 | Myeloid DAP12-associating lectin (MDL-1) | AJ271684 | 1 | 1 345 | 7 736 | 5.8 |
| 32 | Cytochrome C oxidase II (COII; mitochondria) | AF347015 | 19 | 2 555 | 14 572 | 5.7 |
| 33 | β-tubulin | J00314 | 1 | 1 235 | 6 826 | 5.5 |
| 34 | B cell linker protein (BLNK) | NM_013314 | 2 | 1 615 | 8 909 | 5.5 |
| 35 | Activated RNA polymerase II transcription cofactor 4 (PC4) | XM_011218 | 2 | 1 980 | 10 656 | 5.4 |
| 36 | Cytochrome C oxidase I (COI; mitochondria)* | AF347015 | 4 | 2 499 | 13 410 | 5.4 |
| 37 | Enolase 1, α (ENO1) | NM_001428 | 1 | 520 | 2 607 | 5.0 |
| 38 | 90 kD heat shock protein (hsp90) | M16660 | 1 | 2 040 | 10 213 | 5.0 |
| 39 | IL-2Rγ | NM_000206 | 1 | 1 161 | 5 741 | 4.9 |
| 40 | Thymosin β4, X chromosome (TMSB4X) | NM_021109 | 5 | 1 649 | 8 138 | 4.9 |
| 41 | Ferritin, light polypeptide | BC004245 | 3 | 2 527 | 12 327 | 4.9 |
| 42 | NADH dehydrogenase subunit 4 (MTND4; mitochondria) | AF347015 | 1 | 3 738 | 18 023 | 4.8 |
| 43 | Accessory proteins BAP31/BAP29 | NM_005745 | 5 | 966 | 4 598 | 4.8 |
| 44 | Annexin A2 (ANXA2)* | NM_004039 | 8 | 1 221 | 5 506 | 4.5 |
| 45 | Isocitrate dehydrogenase 3 (NAD+) α (IDH3A) | XM_007580 | 1 | 1 754 | 7 766 | 4.4 |
| 46 | Microsomal glutathione S-transferase 2 (MGST2) | XM_003461 | 1 | 1 540 | 6 767 | 4.4 |
| 47 | 12S ribosomal RNA (mitochondria)* | AF347015 | 4 | 1 439 | 6 317 | 4.4 |
| 48 | Cytochrome C oxidase III (COIII; mitochondria)* | AF347015 | 11 | 1 913 | 8 318 | 4.3 |
| 49 | Coagulation factor XIII, A1 polypeptide | AK001685 | 1 | 1 009 | 4 225 | 4.2 |
| 50 | GABA(A) receptor-associated protein (GABARAP) | NM_007278 | 2 | 1 221 | 4 666 | 3.8 |
| 51 | Serine/threonine kinase-15 | BC002499 | 1 | 1 369 | 5 048 | 3.7 |
| 52 | Vesicle-associated membrane protein-8 (VAMP8) | XM_002561 | 2 | 1 976 | 7 267 | 3.7 |
| 53 | Nicastrin | AF240468 | 1 | 1 359 | 4 997 | 3.7 |
| 54 | Purinergic receptor (family A group 5) | XM_007212 | 1 | 1 592 | 5 833 | 3.7 |
| 55 | Rho GDP dissociation inhibitor beta (ARHGDIB) | NM_001175 | 1 | 1 596 | 5 707 | 3.6 |
| 56 | MAD homolog 2 (MADH2) | XM_008795 | 1 | 719 | 2 523 | 3.5 |
| 57 | Cytochrome c, clone MGC: 12367 | BC005299 | 1 | 1 330 | 4 538 | 3.4 |
| 58 | MHC class II HLA-DMα chain | XM_004220 | 1 | 1 403 | 4 552 | 3.2 |
| 59 | High affinity IgE receptor α subunit (FcERI) | X06948 | 1 | 116 | 369 | 3.2 |
| 60 | MLN51 | X80199 | 1 | 1 405 | 4 431 | 3.2 |
| 61 | DORA protein | AJ223183 | 1 | 1 411 | 4 341 | 3.1 |
| 62 | GTP-binding protein (NGB) | XM_005722 | 1 | 877 | 2 674 | 3.0 |
| 63 | Interferon regulatory factor-4 (IRF4) | NM_002460 | 1 | 565 | 1 680 | 3.0 |
| 64 | Crea2 | AL133392 | 2 | 1 437 | 12 646 | 8.8 |
| 65 | Crea8 | BC020516 | 1 | 893 | 3 331 | 3.7 |
| 66 | Crea11 | AA151535 | 1 | 2 639 | 8 446 | 3.2 |
| 67 | Crea12 | AL354813 | 1 | 1 449 | 4 928 | 3.4 |
| 68 | Crea13 | AW295797 | 1 | 1 336 | 9 218 | 6.9 |
| 69 | Crea14 | XM_071675 | 1 | 2 201 | 14 304 | 6.5 |
| Gene no. . | Name . | GenBank accession no. . | Redundancy in screening . | Intensity of BMT . | Intensity of DCs . | DC/BMT . |
|---|---|---|---|---|---|---|
| 1 | α-tubulin* | K00558 | 4 | 1 312 | 37 669 | 28.7 |
| 2 | S100 calcium-binding protein, β (S100B) | NM_006272 | 2 | 1 036 | 28 546 | 27.6 |
| 3 | Matrix metalloproteinase-12 (MMP12)* | XM_006272 | 5 | 1 402 | 38 171 | 27.2 |
| 4 | Thymus and activation-regulated chemokine (TARC) | D43767 | 1 | 1 109 | 26 047 | 23.5 |
| 5 | Myosin phosphatase, target subunit 1 (MYPT1) | XM_006578 | 1 | 2 376 | 43 787 | 18.4 |
| 6 | CD1B antigen (CD1B) | XM_002174 | 4 | 1 286 | 20 646 | 16.1 |
| 7 | CD20-like precursor | NM_022349 | 15 | 1 746 | 27 393 | 15.7 |
| 8 | Ig superfamily protein (Z39Ig)* | XM_010265 | 23 | 1 430 | 18 815 | 13.2 |
| 9 | MHC class II HLA-DQα chain | U77589 | 1 | 1 071 | 13 284 | 12.4 |
| 10 | Glycoprotein NMB (GPNMB) | XM_004781 | 3 | 1 170 | 13 914 | 11.9 |
| 11 | Osteopontin* | NM_000582 | 6 | 2 964 | 33 178 | 11.2 |
| 12 | 5-lipoxygenase activating protein (FLAP) | M63262 | 6 | 1 126 | 12 316 | 10.9 |
| 13 | Mannose receptor C, type 1 (MRC1) | NM_002438 | 3 | 1 284 | 13 960 | 10.9 |
| 14 | Cytochrome b-245, β polypeptide (CYBB) | NM_000397 | 3 | 1 050 | 11 226 | 10.7 |
| 15 | Monocyte chemotactic proteins 4 (MCP4; SCYA13) | XM_008411 | 4 | 1 429 | 14 456 | 10.1 |
| 16 | MHC class II HLA-DRα chain* | XM_004209 | 8 | 1 454 | 14 578 | 10.0 |
| 17 | MHC class II HLA-DRβ chain | M26038 | 6 | 1 607 | 14 838 | 9.2 |
| 18 | RNase A family 1 (RNASE1) | XM_012375 | 2 | 2 413 | 21 588 | 8.9 |
| 19 | Dihydropyrimidinase related protein-2 | D78013 | 4 | 1 997 | 17 249 | 8.6 |
| 20 | ATP synthase 6 (MTATP6; mitochondria) | AF347015 | 11 | 3 394 | 26 657 | 7.9 |
| 21 | 16S ribosomal RNA (mitochondria)* | AF347015 | 6 | 2 683 | 21 015 | 7.8 |
| 22 | Lysosomal acid lipase | U08464 | 22 | 1 043 | 7 756 | 7.4 |
| 23 | Cystatin A (CSTA) | NM_005213 | 4 | 1 286 | 9 437 | 7.3 |
| 24 | Antigen CD36 | M98399 | 8 | 1 404 | 9 501 | 6.8 |
| 25 | Immunoglobulin transcription factor-2 (IFT2) | XM_012756 | 1 | 1 719 | 11 613 | 6.8 |
| 26 | Monocyte chemotactic proteins-1 (MCP1; MCAF) | M24545 | 2 | 2 311 | 14 802 | 6.4 |
| 27 | β-actin | BC004251 | 1 | 715 | 4 561 | 6.4 |
| 28 | Transforming growth factor, β-induced, 68 kD (TGFBI) | NM_000358 | 2 | 1 318 | 8 002 | 6.1 |
| 29 | Cytochrome b (MTCYB; mitochondria)* | AF347015 | 2 | 3 660 | 21 907 | 6.0 |
| 30 | Calmodulin | D45887 | 3 | 752 | 4 383 | 5.8 |
| 31 | Myeloid DAP12-associating lectin (MDL-1) | AJ271684 | 1 | 1 345 | 7 736 | 5.8 |
| 32 | Cytochrome C oxidase II (COII; mitochondria) | AF347015 | 19 | 2 555 | 14 572 | 5.7 |
| 33 | β-tubulin | J00314 | 1 | 1 235 | 6 826 | 5.5 |
| 34 | B cell linker protein (BLNK) | NM_013314 | 2 | 1 615 | 8 909 | 5.5 |
| 35 | Activated RNA polymerase II transcription cofactor 4 (PC4) | XM_011218 | 2 | 1 980 | 10 656 | 5.4 |
| 36 | Cytochrome C oxidase I (COI; mitochondria)* | AF347015 | 4 | 2 499 | 13 410 | 5.4 |
| 37 | Enolase 1, α (ENO1) | NM_001428 | 1 | 520 | 2 607 | 5.0 |
| 38 | 90 kD heat shock protein (hsp90) | M16660 | 1 | 2 040 | 10 213 | 5.0 |
| 39 | IL-2Rγ | NM_000206 | 1 | 1 161 | 5 741 | 4.9 |
| 40 | Thymosin β4, X chromosome (TMSB4X) | NM_021109 | 5 | 1 649 | 8 138 | 4.9 |
| 41 | Ferritin, light polypeptide | BC004245 | 3 | 2 527 | 12 327 | 4.9 |
| 42 | NADH dehydrogenase subunit 4 (MTND4; mitochondria) | AF347015 | 1 | 3 738 | 18 023 | 4.8 |
| 43 | Accessory proteins BAP31/BAP29 | NM_005745 | 5 | 966 | 4 598 | 4.8 |
| 44 | Annexin A2 (ANXA2)* | NM_004039 | 8 | 1 221 | 5 506 | 4.5 |
| 45 | Isocitrate dehydrogenase 3 (NAD+) α (IDH3A) | XM_007580 | 1 | 1 754 | 7 766 | 4.4 |
| 46 | Microsomal glutathione S-transferase 2 (MGST2) | XM_003461 | 1 | 1 540 | 6 767 | 4.4 |
| 47 | 12S ribosomal RNA (mitochondria)* | AF347015 | 4 | 1 439 | 6 317 | 4.4 |
| 48 | Cytochrome C oxidase III (COIII; mitochondria)* | AF347015 | 11 | 1 913 | 8 318 | 4.3 |
| 49 | Coagulation factor XIII, A1 polypeptide | AK001685 | 1 | 1 009 | 4 225 | 4.2 |
| 50 | GABA(A) receptor-associated protein (GABARAP) | NM_007278 | 2 | 1 221 | 4 666 | 3.8 |
| 51 | Serine/threonine kinase-15 | BC002499 | 1 | 1 369 | 5 048 | 3.7 |
| 52 | Vesicle-associated membrane protein-8 (VAMP8) | XM_002561 | 2 | 1 976 | 7 267 | 3.7 |
| 53 | Nicastrin | AF240468 | 1 | 1 359 | 4 997 | 3.7 |
| 54 | Purinergic receptor (family A group 5) | XM_007212 | 1 | 1 592 | 5 833 | 3.7 |
| 55 | Rho GDP dissociation inhibitor beta (ARHGDIB) | NM_001175 | 1 | 1 596 | 5 707 | 3.6 |
| 56 | MAD homolog 2 (MADH2) | XM_008795 | 1 | 719 | 2 523 | 3.5 |
| 57 | Cytochrome c, clone MGC: 12367 | BC005299 | 1 | 1 330 | 4 538 | 3.4 |
| 58 | MHC class II HLA-DMα chain | XM_004220 | 1 | 1 403 | 4 552 | 3.2 |
| 59 | High affinity IgE receptor α subunit (FcERI) | X06948 | 1 | 116 | 369 | 3.2 |
| 60 | MLN51 | X80199 | 1 | 1 405 | 4 431 | 3.2 |
| 61 | DORA protein | AJ223183 | 1 | 1 411 | 4 341 | 3.1 |
| 62 | GTP-binding protein (NGB) | XM_005722 | 1 | 877 | 2 674 | 3.0 |
| 63 | Interferon regulatory factor-4 (IRF4) | NM_002460 | 1 | 565 | 1 680 | 3.0 |
| 64 | Crea2 | AL133392 | 2 | 1 437 | 12 646 | 8.8 |
| 65 | Crea8 | BC020516 | 1 | 893 | 3 331 | 3.7 |
| 66 | Crea11 | AA151535 | 1 | 2 639 | 8 446 | 3.2 |
| 67 | Crea12 | AL354813 | 1 | 1 449 | 4 928 | 3.4 |
| 68 | Crea13 | AW295797 | 1 | 1 336 | 9 218 | 6.9 |
| 69 | Crea14 | XM_071675 | 1 | 2 201 | 14 304 | 6.5 |
DC-associated clones identified by a series of microarray assays are listed together with their GenBank accession numbers. Redundancy represents the number of identical clones among the selected 374 cDNAs. Intensity of BMT and DC means the average signal intensity of each clone at the laser scan of the microarray after normalization and background subtraction. DC/BMT represents the ratio of fluorescence intensity as determined by forward- (DC-BMT) and backward- (BMT-DC) subtracted probes.
DNA clones used for the screening-out experiments to minimize the number of redundant clones.