MMP/TIMP gene and protein expression in RMS cell lines without (control, c) and after (SDF) SDF-1 stimulation
| Cell line . | MMP-9 . | MMP-2 . | TIMP-1 . | TIMP-2 . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Gene . | Protein . | Gene . | Protein . | Protein . | Protein . | |||||||
| c . | SDF . | c . | SDF . | c . | SDF . | c . | SDF . | c . | SDF . | c . | SDF . | |
| RH5 | − | − | − | − | + | ++ | + | ++ | + | < | + | < |
| RH28 | − | ND | − | − | + | + | + | ++ | + | < | + | < |
| RH30 | − | − | − | − | + | + | + | + | + | + | + | < |
| RHRKMP4 | + | + | + | + | + | + | + | + | + | < | + | < |
| CW9019 | + | + | + | + | + | + | + | + | + | < | + | < |
| RD | + | + | + | + | + | + | + | + | + | < | + | < |
| SMS-CTR | + | + | + | + | + | + | + | + | + | ++ | + | + |
| Cell line . | MMP-9 . | MMP-2 . | TIMP-1 . | TIMP-2 . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Gene . | Protein . | Gene . | Protein . | Protein . | Protein . | |||||||
| c . | SDF . | c . | SDF . | c . | SDF . | c . | SDF . | c . | SDF . | c . | SDF . | |
| RH5 | − | − | − | − | + | ++ | + | ++ | + | < | + | < |
| RH28 | − | ND | − | − | + | + | + | ++ | + | < | + | < |
| RH30 | − | − | − | − | + | + | + | + | + | + | + | < |
| RHRKMP4 | + | + | + | + | + | + | + | + | + | < | + | < |
| CW9019 | + | + | + | + | + | + | + | + | + | < | + | < |
| RD | + | + | + | + | + | + | + | + | + | < | + | < |
| SMS-CTR | + | + | + | + | + | + | + | + | + | ++ | + | + |
− indicates lack of transcripts/protein; +, presence of transcripts/protein; ++, 1.5-fold increase and greater; <, 1.5-fold decrease and greater; ND, not done.