Parameters characterizing the movement of CW9019, RH30 ARMS, and SMS-CTR ERMS cells and A204 sarcoma cells in control medium (—) and in the presence of SDF-1β
| Cell line . | TLcT . | AScM . | TLcD . | AvcD . | CME . |
|---|---|---|---|---|---|
| CW9019 | |||||
| (—) | 130.21 ± 2.33 | 0.65 ± 0.01 | 39.58 ± 2.44 | 0.20 ± 0.01 | 0.31 ± 0.02 |
| SDF-1 | 175.46 ± 4.51* | 0.88 ± 0.02* | 55.26 ± 3.44* | 0.28 ± 0.02* | 0.32 ± 0.02 |
| RH30 | |||||
| (—) | 167.00 ± 4.27 | 0.83 ± 0.02 | 46.75 ± 3.91 | 0.23 ± 0.02 | 0.28 ± 0.02 |
| SDF-1 | 184.50 ± 5.73* | 0.92 ± 0.03* | 64.72 ± 3.87 | 0.32 ± 0.02 | 0.35 ± 0.02 |
| SMS-CTR | |||||
| (—) | 134.80 ± 7.46 | 0.67 ± 0.04 | 45.98 ± 3.12 | 0.23 ± 0.02 | 0.37 ± 0.02 |
| SDF-1 | 184.65 ± 6.73* | 0.92 ± 0.03* | 94.08 ± 7.43* | 0.47 ± 0.04* | 0.50 ± 0.03* |
| A204 | |||||
| (—) | 95.45 ± 2.35 | 0.48 ± 0.01 | 16.60 ± 1.42 | 0.08 ± 0.01 | 0.17 ± 0.01 |
| SDF-1 | 110.99 ± 3.05 | 0.55 ± 0.02 | 19.28 ± 1.49 | 0.09 ± 0.01 | 0.17 ± 0.01 |
| Cell line . | TLcT . | AScM . | TLcD . | AvcD . | CME . |
|---|---|---|---|---|---|
| CW9019 | |||||
| (—) | 130.21 ± 2.33 | 0.65 ± 0.01 | 39.58 ± 2.44 | 0.20 ± 0.01 | 0.31 ± 0.02 |
| SDF-1 | 175.46 ± 4.51* | 0.88 ± 0.02* | 55.26 ± 3.44* | 0.28 ± 0.02* | 0.32 ± 0.02 |
| RH30 | |||||
| (—) | 167.00 ± 4.27 | 0.83 ± 0.02 | 46.75 ± 3.91 | 0.23 ± 0.02 | 0.28 ± 0.02 |
| SDF-1 | 184.50 ± 5.73* | 0.92 ± 0.03* | 64.72 ± 3.87 | 0.32 ± 0.02 | 0.35 ± 0.02 |
| SMS-CTR | |||||
| (—) | 134.80 ± 7.46 | 0.67 ± 0.04 | 45.98 ± 3.12 | 0.23 ± 0.02 | 0.37 ± 0.02 |
| SDF-1 | 184.65 ± 6.73* | 0.92 ± 0.03* | 94.08 ± 7.43* | 0.47 ± 0.04* | 0.50 ± 0.03* |
| A204 | |||||
| (—) | 95.45 ± 2.35 | 0.48 ± 0.01 | 16.60 ± 1.42 | 0.08 ± 0.01 | 0.17 ± 0.01 |
| SDF-1 | 110.99 ± 3.05 | 0.55 ± 0.02 | 19.28 ± 1.49 | 0.09 ± 0.01 | 0.17 ± 0.01 |
Values are given as the mean ± SEM.
Statistically significant at P < .05.
TLcT indicates total length of cell trajectory (μm); AScM, average speed of cell movement (μm/min)—the average speed of cell; TLcD, total length of cell displacement (μm); AvcD, average velocity of cell displacement (μm/min)—the average velocity of cell displacement is defined as length of final cell displacement/time of recording; CME, coefficient of movement efficiency—the ratio of cell displacement to cell trajectory length. CME would equal 1 for cells moving persistently along one straight line in one direction and 0 for random movement.