Cell-cycle progression of FA-D2 transfectants
| Treatment . | Time after release (h) . | PD20-FANCD2 (wt) . | PD20-FANCD2 (K561R) . | PD20-pMMP . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| G1 . | S . | G2/M . | G1 . | S . | G2/M . | G1 . | S . | G2/M . | ||
| −MMC | 0 | 83.7 | 12.4 | 3.9 | 79.1 | 16.0 | 4.9 | 81.4 | 12.8 | 5.8 |
| 3 | 2.9 | 86.0 | 11.1 | 0.0 | 94.2 | 5.8 | 9.8 | 87.3 | 2.9 | |
| 8 | 2.0 | 16.0 | 82.0 | 2.6 | 18.3 | 79.1 | 5.1 | 10.1 | 84.8 | |
| 12 | 42.3 | 10.1 | 47.6 | 45.4 | 8.4 | 46.2 | 45.6 | 11.1 | 43.3 | |
| +MMC | 0 | 79.2 | 18.7 | 2.1 | 81.9 | 14.7 | 3.4 | 80.6 | 14.9 | 4.5 |
| 3 | 1.7 | 87.0 | 11.3 | 9.7 | 85.7 | 4.6 | 2.3 | 88.0 | 9.7 | |
| 8 | 2.2 | 16.1 | 81.7 | 2.1 | 20.1 | 77.8 | 2.5 | 20.4 | 77.1 | |
| 12 | 5.4 | 11.4 | 83.2 | 2.9 | 9.1 | 88.1 | 3.8 | 9.0 | 87.2 | |
| Treatment . | Time after release (h) . | PD20-FANCD2 (wt) . | PD20-FANCD2 (K561R) . | PD20-pMMP . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| G1 . | S . | G2/M . | G1 . | S . | G2/M . | G1 . | S . | G2/M . | ||
| −MMC | 0 | 83.7 | 12.4 | 3.9 | 79.1 | 16.0 | 4.9 | 81.4 | 12.8 | 5.8 |
| 3 | 2.9 | 86.0 | 11.1 | 0.0 | 94.2 | 5.8 | 9.8 | 87.3 | 2.9 | |
| 8 | 2.0 | 16.0 | 82.0 | 2.6 | 18.3 | 79.1 | 5.1 | 10.1 | 84.8 | |
| 12 | 42.3 | 10.1 | 47.6 | 45.4 | 8.4 | 46.2 | 45.6 | 11.1 | 43.3 | |
| +MMC | 0 | 79.2 | 18.7 | 2.1 | 81.9 | 14.7 | 3.4 | 80.6 | 14.9 | 4.5 |
| 3 | 1.7 | 87.0 | 11.3 | 9.7 | 85.7 | 4.6 | 2.3 | 88.0 | 9.7 | |
| 8 | 2.2 | 16.1 | 81.7 | 2.1 | 20.1 | 77.8 | 2.5 | 20.4 | 77.1 | |
| 12 | 5.4 | 11.4 | 83.2 | 2.9 | 9.1 | 88.1 | 3.8 | 9.0 | 87.2 | |
Indicated PD20 (FA-D2) fibroblast transfectants were arrested at G1/S using double-thymidine block and were released into S phase.
Cell-cycle progression was analyzed by DNA flow histograms of propidium iodide–stained cells.