Genotype of transgene-rescued EpoR mutant mouse offspring
| Crossing . | Litters . | Pups . | Transgene . | EpoRgenotype . | ||
|---|---|---|---|---|---|---|
| +/+ (%) . | +/− (%) . | −/− (%) . | ||||
| EpoR+/−∷Tg-A andEpoR+/− | 8 | 90 | + | 15 (24) | 28 (45) | 19 (31) |
| − | 6 (21) | 22 (79) | 0 (0) | |||
| EpoR+/−∷Tg-B andEpoR+/− | 7 | 49 | + | 5 (17) | 16 (53) | 9 (30) |
| − | 4 (21) | 15 (79) | 0 (0) | |||
| EpoR−/−∷Tg-B andEpoR−/−∷Tg-B | 4 | 23* | + | — | — | 23 (100) |
| − | — | — | 0 (0) | |||
| Crossing . | Litters . | Pups . | Transgene . | EpoRgenotype . | ||
|---|---|---|---|---|---|---|
| +/+ (%) . | +/− (%) . | −/− (%) . | ||||
| EpoR+/−∷Tg-A andEpoR+/− | 8 | 90 | + | 15 (24) | 28 (45) | 19 (31) |
| − | 6 (21) | 22 (79) | 0 (0) | |||
| EpoR+/−∷Tg-B andEpoR+/− | 7 | 49 | + | 5 (17) | 16 (53) | 9 (30) |
| − | 4 (21) | 15 (79) | 0 (0) | |||
| EpoR−/−∷Tg-B andEpoR−/−∷Tg-B | 4 | 23* | + | — | — | 23 (100) |
| − | — | — | 0 (0) | |||
Two lines of mice containing the HG1-EpoR transgene (Tg-A and Tg-B) were used for the rescue experiments. The genotypes of pups derived from each parent were determined 3 to 4 weeks after birth by PCR, Southern blot analysis, or both.
Twelve male and 11 female pups were derived from the interbreeding of Rescued-B mice.