Table 1. Primer and probe sequences... | American Society of Hematology

Table 1.

Primer and probe sequences

Oligonucleotide	Position^*	Sequence 5′ → 3′	Melting temperature
Forward primer	1816-1837	GCA ACT TTT TCA CCT CTG CCT A	T_m 57°C
TaqMan probe	1863-1892	VIC-TTC AAG CCT CCA AGC TGT GCC TTG GGT GGC-TAMRA	T_m 78°C
Reverse primer	1923-1947	AGT AAC TCC ACA GTA GCT CCA AAT T	T_m 56°C

Oligonucleotide	Position^*	Sequence 5′ → 3′	Melting temperature
Forward primer	1816-1837	GCA ACT TTT TCA CCT CTG CCT A	T_m 57°C
TaqMan probe	1863-1892	VIC-TTC AAG CCT CCA AGC TGT GCC TTG GGT GGC-TAMRA	T_m 78°C
Reverse primer	1923-1947	AGT AAC TCC ACA GTA GCT CCA AAT T	T_m 56°C

Numbering starts at the unique EcoRI site at position 3221/0.