Quantification of the morphological abnormality of neutrophils differentiated in vitro from the transduced bone marrow cells
| . | % Abnormal neutrophils . | |||
|---|---|---|---|---|
| Vector* . | Rac2D57N† . | Rac2D57NΔCT* . | Rac2D57NΔM* . | |
| Experiment 1 | 16.6 (223) | 67.7 (204) | 10.2 (235) | 12.6 (222) |
| Experiment 2 | 10.1 (228) | 73.0 (222) | 9.7 (237) | 11.0 (228) |
| Experiment 3 | 11.8 (220) | 62.4 (218) | 10.3 (234) | 12.3 (245) |
| . | % Abnormal neutrophils . | |||
|---|---|---|---|---|
| Vector* . | Rac2D57N† . | Rac2D57NΔCT* . | Rac2D57NΔM* . | |
| Experiment 1 | 16.6 (223) | 67.7 (204) | 10.2 (235) | 12.6 (222) |
| Experiment 2 | 10.1 (228) | 73.0 (222) | 9.7 (237) | 11.0 (228) |
| Experiment 3 | 11.8 (220) | 62.4 (218) | 10.3 (234) | 12.3 (245) |
The numbers in parentheses indicate the total number of cells counted.
The defects in these neutrophils are relatively mild, consisting only of hypersegmented nucleus (bi-nucleated cells were occasionally found).
The defects in these neutrophils are much more severe. They include multiple nuclei, hypersegmented nuclei, much larger cell sizes, intense granules and abnormal granulation, and asynchronous nuclear and cytoplasmic maturation.