Primers used in RT-PCR
| . | Forward primer . | Reverse primer . | Product size, base pairs . |
|---|---|---|---|
| Primers for RT-PCR | |||
| HAS1 | 5′-GTGAGTGGCTGTACAACGCG-3′ | 5′-AGAGGGACGTAGTTAGCGGC-3′ | 354 |
| HAS2 | 5′-TGGCATCACACCTCATCATC-3′ | 5′-ACCAATTGCGTTACGTGTTG-3′ | 368 |
| HAS3 | 5′-TTGGCTGTGTGCAGTGTATTAGT-3′ | 5′-GGTCTCTGTGAGGCACTTGG-3′ | 200 |
| β-actin | 5′-TCCCTGGAGAAGAGCTACGA-3′ | 5′-AGCACTGTGTTGGCGTACAG-3′ | 194 |
| Insert primers for competitive RT-PCR | |||
| HAS1 forward | 5′-TAATACGACTCACTATAGGGTGAGTGGCTGTACAACGCGTCCCTGGAGAAGAGCTACGA-3′ | ||
| HAS1 reverse | 5′-TTTTTTTTTTTTTTTTTTAGAGGGACGTAGTTAGCGGCAGCACTGTGTTGGCGTACCG-3′ | 194 | |
| HAS2 forward | 5′-TAATACGACTCACTATAGGTGGCATCACACCTCATCATCTCCCTGGAGAAGAGCTACGA-3′ | ||
| HAS2 reverse | 5′-TTTTTTTTTTTTTTTTTTACCAATTGCGTTACGTGTTGAGCACTGTGTTGGCGTACCG-3′ | 194 | |
| . | Forward primer . | Reverse primer . | Product size, base pairs . |
|---|---|---|---|
| Primers for RT-PCR | |||
| HAS1 | 5′-GTGAGTGGCTGTACAACGCG-3′ | 5′-AGAGGGACGTAGTTAGCGGC-3′ | 354 |
| HAS2 | 5′-TGGCATCACACCTCATCATC-3′ | 5′-ACCAATTGCGTTACGTGTTG-3′ | 368 |
| HAS3 | 5′-TTGGCTGTGTGCAGTGTATTAGT-3′ | 5′-GGTCTCTGTGAGGCACTTGG-3′ | 200 |
| β-actin | 5′-TCCCTGGAGAAGAGCTACGA-3′ | 5′-AGCACTGTGTTGGCGTACAG-3′ | 194 |
| Insert primers for competitive RT-PCR | |||
| HAS1 forward | 5′-TAATACGACTCACTATAGGGTGAGTGGCTGTACAACGCGTCCCTGGAGAAGAGCTACGA-3′ | ||
| HAS1 reverse | 5′-TTTTTTTTTTTTTTTTTTAGAGGGACGTAGTTAGCGGCAGCACTGTGTTGGCGTACCG-3′ | 194 | |
| HAS2 forward | 5′-TAATACGACTCACTATAGGTGGCATCACACCTCATCATCTCCCTGGAGAAGAGCTACGA-3′ | ||
| HAS2 reverse | 5′-TTTTTTTTTTTTTTTTTTACCAATTGCGTTACGTGTTGAGCACTGTGTTGGCGTACCG-3′ | 194 | |