Primers and conditions for the RT-PCR experiments performed in this study
| Gene . | Sequence of primers (5′ → 3′) . | T (°C) . | Product . |
|---|---|---|---|
| IL-2 | GCAACTCCTGTCTTGCATTG | 59 | 173 |
| AATGTGAGCATCCTGGTGAG | |||
| IFN-γ | AGCTCTGCATCGTTTTGGGTTC | 64 | 492 |
| CAAATATTGCAGGCAGGACAACC | |||
| IL-3 | GCTCCCATGACCCAGACAACGTCC | 61 | 400 |
| CTAAAAGATCGCGAGGCTCAAAGTCGT | |||
| IL-4 | CTGTGCTCCGGCAGTTCTAC | 58 | 176 |
| ACGTACTCTGGTTGGCTTCC | |||
| IL-6 | GCCAGAGCTGTGCAGATGAG | 58 | 187 |
| AGGAACTCCTTAAAGCTGCG | |||
| IL-10 | ACCCAGTCTGAGAACAGCTGC | 61 | 260 |
| GTTCACATGCGCCTTGATGTCT | |||
| IL-13 | GACCACGGTCATTGCTCTCACT | 61 | 234 |
| TCTGGGTCTTCTCGATGGCACT | |||
| β2m | CCCCCACTGAAAAAGATGAG | 56 | 150 |
| TCACTCAATCCAAATGCGGC |
| Gene . | Sequence of primers (5′ → 3′) . | T (°C) . | Product . |
|---|---|---|---|
| IL-2 | GCAACTCCTGTCTTGCATTG | 59 | 173 |
| AATGTGAGCATCCTGGTGAG | |||
| IFN-γ | AGCTCTGCATCGTTTTGGGTTC | 64 | 492 |
| CAAATATTGCAGGCAGGACAACC | |||
| IL-3 | GCTCCCATGACCCAGACAACGTCC | 61 | 400 |
| CTAAAAGATCGCGAGGCTCAAAGTCGT | |||
| IL-4 | CTGTGCTCCGGCAGTTCTAC | 58 | 176 |
| ACGTACTCTGGTTGGCTTCC | |||
| IL-6 | GCCAGAGCTGTGCAGATGAG | 58 | 187 |
| AGGAACTCCTTAAAGCTGCG | |||
| IL-10 | ACCCAGTCTGAGAACAGCTGC | 61 | 260 |
| GTTCACATGCGCCTTGATGTCT | |||
| IL-13 | GACCACGGTCATTGCTCTCACT | 61 | 234 |
| TCTGGGTCTTCTCGATGGCACT | |||
| β2m | CCCCCACTGAAAAAGATGAG | 56 | 150 |
| TCACTCAATCCAAATGCGGC |
T (°C) indicates annealing temperature; product, PCR product size (bp).