Table 2.

Results of mass spectrometric analysis of 2-DE spot 25 identified as κ light chain

Mass (Da)Theoretical. mass (Da)SequencesFrom*
1860.98† 1860.930 DIQLTQSPSSLSASVGDR 001 -018  
1749.98† 1749.938 LLIYDASNVETGVLSR 046 -061  
1579.76† 1579.766 FSGSGSGTFYSLTIR 062 -076  
3141.70 3141.451 SLQPEDIATYYCac°QQYQDFPLTFGGGTK 077 -103  
2102.05 2102.129 RTVAAPSVFIFPPSDEQLK 108 -126 
1946.04 1946.027 TVAAPSVFIFPPSDEQLK 109 -126 
1811.92 1811.911 SGTASVVCac°LLNNFYPR 127 -142 
1503.80 1503.758 DSTYSLSSTLTLSK 170 -183 
1903.96 1903.958 LYACac°EVTHQGLSSPVTK 191 -207 
Mass (Da)Theoretical. mass (Da)SequencesFrom*
1860.98† 1860.930 DIQLTQSPSSLSASVGDR 001 -018  
1749.98† 1749.938 LLIYDASNVETGVLSR 046 -061  
1579.76† 1579.766 FSGSGSGTFYSLTIR 062 -076  
3141.70 3141.451 SLQPEDIATYYCac°QQYQDFPLTFGGGTK 077 -103  
2102.05 2102.129 RTVAAPSVFIFPPSDEQLK 108 -126 
1946.04 1946.027 TVAAPSVFIFPPSDEQLK 109 -126 
1811.92 1811.911 SGTASVVCac°LLNNFYPR 127 -142 
1503.80 1503.758 DSTYSLSSTLTLSK 170 -183 
1903.96 1903.958 LYACac°EVTHQGLSSPVTK 191 -207 

Cac° indicates cysteine modified with acrylamide.

*

Residues are numbered according to the standard Kabat numbering system.77 

†

Mass peaks identified in all spots under investigation.

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