Sequences of primers
| Name . | Sequence . | Remark . |
|---|---|---|
| spdT | 5′-GAGCAAATTCCTGTACTGAC (T)30 NN-3′ | Production of cDNA |
| MC18 | 5′-GAGCAAATTCCTGTACTGAC-3′ | Specific part of spdT |
| 3113 | 5′-CGGATAACAATTTCACACAGG-3′ | UniversallacZ reversed |
| 6142 | 5′-CGGGCTGCAGGCGGGATCCTACACCTGGAG-3′ | Exon 3 forward |
| 6277 | 5′-AATGGTGACTCCCAGGTCGAG-3′ | Exon 6 reversed |
| 6278 | 5′-CGCTCCCTGGTGGAGCTGACC-3′ | Exon 11 forward |
| 6346 | 5′-ATCATGAAGCGTGGAGACAGC-3′ | Exon 13 forward |
| 6351 | 5′-GAGTTGCCTGATGGTAACCG-3′ | Exon 6 forward |
| 6395 | 5′-CCTGGAGGGGTCCCCAGATG-3′ | Exon 16 reversed |
| 6406 | 5′-TGCAGCCCACGGAAGGGCTC-3′ | Exon 14 forward |
| 6407 | 5′-GAGCCCTTCCGTGGGCTGCA-3′ | Exon 14 reversed |
| 6480 | 5′-CATCCTGCGTCTGGACCTGGCTG-3′ | β-Actin forward |
| 6482 | 5′-GACCTGGCCGTCAGGCAGCTCG-3′ | β-Actin reversed |
| 6506 | 5′-CAGGGCTCCAGGCTGCAGGC-3′ | Exon 23 reversed |
| 6546 | 5′-TGGAGGTCAGCACCAACACA-3′ | Exon 11 reversed |
| 6548 | 5′-AGGAAGAGCTGTGTGGCCTG-3′ | Exon 22 forward |
| 6601 | 5′-AGCGAATTCGCTGCAGGCGGCATCCTACACC-3′ | Exon 3 forwardEcoRl |
| 6617 | 5′-AGCCTCGAGCTGGCCAGACACGGAACAAAT-3′ | 3′ untranslated reversed Xhol |
| 6650 | 5′-AGCCTCGAGGGTTCCTTCCTTTCCCTTCCAGGA-3′ | Exon 29 reversed |
| 6727 | 5′-CTGCTGGAAATGGGAGGGTC-3′ | Exon 1 reversed |
| 6728 | 5′-CCGCCTCTGCCTCTGCCTCT-3′ | Exon 2 reversed |
| 6753 | 5′-GACATGTTGCTGCTTTGGGGC-3′ | Exon 27 forward |
| 6839 | 5′-GATCGAATTCGCCGGCCACCATGCACCAGCGTCACCCCCG-3′ | Exon 1 forward EcoRl |
| Name . | Sequence . | Remark . |
|---|---|---|
| spdT | 5′-GAGCAAATTCCTGTACTGAC (T)30 NN-3′ | Production of cDNA |
| MC18 | 5′-GAGCAAATTCCTGTACTGAC-3′ | Specific part of spdT |
| 3113 | 5′-CGGATAACAATTTCACACAGG-3′ | UniversallacZ reversed |
| 6142 | 5′-CGGGCTGCAGGCGGGATCCTACACCTGGAG-3′ | Exon 3 forward |
| 6277 | 5′-AATGGTGACTCCCAGGTCGAG-3′ | Exon 6 reversed |
| 6278 | 5′-CGCTCCCTGGTGGAGCTGACC-3′ | Exon 11 forward |
| 6346 | 5′-ATCATGAAGCGTGGAGACAGC-3′ | Exon 13 forward |
| 6351 | 5′-GAGTTGCCTGATGGTAACCG-3′ | Exon 6 forward |
| 6395 | 5′-CCTGGAGGGGTCCCCAGATG-3′ | Exon 16 reversed |
| 6406 | 5′-TGCAGCCCACGGAAGGGCTC-3′ | Exon 14 forward |
| 6407 | 5′-GAGCCCTTCCGTGGGCTGCA-3′ | Exon 14 reversed |
| 6480 | 5′-CATCCTGCGTCTGGACCTGGCTG-3′ | β-Actin forward |
| 6482 | 5′-GACCTGGCCGTCAGGCAGCTCG-3′ | β-Actin reversed |
| 6506 | 5′-CAGGGCTCCAGGCTGCAGGC-3′ | Exon 23 reversed |
| 6546 | 5′-TGGAGGTCAGCACCAACACA-3′ | Exon 11 reversed |
| 6548 | 5′-AGGAAGAGCTGTGTGGCCTG-3′ | Exon 22 forward |
| 6601 | 5′-AGCGAATTCGCTGCAGGCGGCATCCTACACC-3′ | Exon 3 forwardEcoRl |
| 6617 | 5′-AGCCTCGAGCTGGCCAGACACGGAACAAAT-3′ | 3′ untranslated reversed Xhol |
| 6650 | 5′-AGCCTCGAGGGTTCCTTCCTTTCCCTTCCAGGA-3′ | Exon 29 reversed |
| 6727 | 5′-CTGCTGGAAATGGGAGGGTC-3′ | Exon 1 reversed |
| 6728 | 5′-CCGCCTCTGCCTCTGCCTCT-3′ | Exon 2 reversed |
| 6753 | 5′-GACATGTTGCTGCTTTGGGGC-3′ | Exon 27 forward |
| 6839 | 5′-GATCGAATTCGCCGGCCACCATGCACCAGCGTCACCCCCG-3′ | Exon 1 forward EcoRl |