In vitro cytokine-stimulated cell expansion from sorted KLS cells
| Cytokine cocktail . | WT KLS 6d-fold expansion . | STAT5ab−/−KLS 6d-fold expansion . | STAT5ab−/− -fold reduction . | P . |
|---|---|---|---|---|
| IL36S | 1164 ± 213 | 138 ± 116 | 8.4 | <.01 |
| IL3SFT | 1012 ± 624 | 79 ± 81 | 12.8 | .05 |
| IL3S | 749 ± 455 | 73 ± 43 | 10.3 | .05 |
| IL3F | 182 ± 40 | 85 ± 74 | 2.1 | .12 |
| IL3T | 213 ± 182 | 39 ± 19 | 5.5 | .16 |
| SFT | 76 ± 26 | 22 ± 21 | 3.5 | .02 |
| Cytokine cocktail . | WT KLS 6d-fold expansion . | STAT5ab−/−KLS 6d-fold expansion . | STAT5ab−/− -fold reduction . | P . |
|---|---|---|---|---|
| IL36S | 1164 ± 213 | 138 ± 116 | 8.4 | <.01 |
| IL3SFT | 1012 ± 624 | 79 ± 81 | 12.8 | .05 |
| IL3S | 749 ± 455 | 73 ± 43 | 10.3 | .05 |
| IL3F | 182 ± 40 | 85 ± 74 | 2.1 | .12 |
| IL3T | 213 ± 182 | 39 ± 19 | 5.5 | .16 |
| SFT | 76 ± 26 | 22 ± 21 | 3.5 | .02 |
KLS cells were sorted by FACS and plated in 96-well plates in the presence of various cytokine cocktails. After 6 days in culture, the total nucleated cell count was determined using a hemacytometer. The fold cell expansion was calculated based on the starting number of cells seeded per well.
IL3 indicates murine interleukin 3 (20 ng/mL); 6, human interleukin 6 (50 ng/mL); S, murine stem cell factor (50 ng/mL); F, murine flt-3 ligand (50 ng/mL); and T, murine thrombopoietin (50 ng/mL). The results are the averages ± SD for 4 to 6 experiments.