Analysis of mice reconstituted with WT and TGF-β1−/− marrow cells infected with the MIGR-TPO virus
| Tissue . | Items . | Wild-type control . | Week 6 after transplantation . | Week 16 after transplantation . | ||
|---|---|---|---|---|---|---|
| WT . | TGF-β1−/− . | WT . | TGF-β1−/− . | |||
| Bone marrow | Cells/femur, ×106 | 29.2 ± 3.3 | 16.2 ± 2.7 | 16.5 ± 3.5 | 12.4 ± 4.2 | 10.1 ± 0.3 |
| CFCs/femur, ×103 | 86.5 ± 8.3 | 49.2 ± 7.4 | 48.6 ± 9.1 | 15.1 ± 5.2 | 26.3 ± 3.7 | |
| Spleen | Weight, mg | 126 ± 20 | 595 ± 88 | 1025 ± 225 | 471 ± 1263-150 | 173 ± 683-150 |
| CFCs/106cells | 88 ± 22 | 1280 ± 123 | 1845 ± 250 | 385 ± 112 | 1230 ± 759 | |
| Blood | Nucleated cells/mL, ×106 | 9.6 ± 0.7 | 70.5 ± 30.6 | 67.5 ± 31.5 | 41.8 ± 11.1 | 41.3 ± 29.2 |
| CFCs/mL, ×102 | < 1 | 1100 ± 260 | 590 ± 100 | 200 ± 86 | 360 ± 140 | |
| Tissue . | Items . | Wild-type control . | Week 6 after transplantation . | Week 16 after transplantation . | ||
|---|---|---|---|---|---|---|
| WT . | TGF-β1−/− . | WT . | TGF-β1−/− . | |||
| Bone marrow | Cells/femur, ×106 | 29.2 ± 3.3 | 16.2 ± 2.7 | 16.5 ± 3.5 | 12.4 ± 4.2 | 10.1 ± 0.3 |
| CFCs/femur, ×103 | 86.5 ± 8.3 | 49.2 ± 7.4 | 48.6 ± 9.1 | 15.1 ± 5.2 | 26.3 ± 3.7 | |
| Spleen | Weight, mg | 126 ± 20 | 595 ± 88 | 1025 ± 225 | 471 ± 1263-150 | 173 ± 683-150 |
| CFCs/106cells | 88 ± 22 | 1280 ± 123 | 1845 ± 250 | 385 ± 112 | 1230 ± 759 | |
| Blood | Nucleated cells/mL, ×106 | 9.6 ± 0.7 | 70.5 ± 30.6 | 67.5 ± 31.5 | 41.8 ± 11.1 | 41.3 ± 29.2 |
| CFCs/mL, ×102 | < 1 | 1100 ± 260 | 590 ± 100 | 200 ± 86 | 360 ± 140 | |
Values are means ± SD of data from 3 controls and 3 mice in each group. Progenitor cells numbers (CFCs) were calculated from the number of colonies obtained from 1 × 105cells grown in semisolid medium.
Statistically not significant. P < .1.