Chimerism of wild-type hosts reconstituted with marrow transplants from TGF-β1−/− donors
| Time after transplantation . | Mouse no. . | Neo/actin . | % chimerism . |
|---|---|---|---|
| Week 6 | KO9 | 21/25 | 84 |
| KO10 | 25/28 | 89 | |
| KO12 | 17/25 | 70 | |
| Week 16 | KO1 | 25/39 | 64 |
| KO7 | 5/22 | 22 | |
| KO8 | 21/28 | 75 | |
| KO14 | 30/30 | 100 | |
| KO11 | 30/30 | 100 |
| Time after transplantation . | Mouse no. . | Neo/actin . | % chimerism . |
|---|---|---|---|
| Week 6 | KO9 | 21/25 | 84 |
| KO10 | 25/28 | 89 | |
| KO12 | 17/25 | 70 | |
| Week 16 | KO1 | 25/39 | 64 |
| KO7 | 5/22 | 22 | |
| KO8 | 21/28 | 75 | |
| KO14 | 30/30 | 100 | |
| KO11 | 30/30 | 100 |
Irradiated WT hosts were engrafted with virus-infected marrow cells from TGF-β1−/− donors (KO). Marrow CFC-derived colonies (30 per recipient animal) were picked, and samples were analyzed by PCR with primers specific for theneo gene indicative of the mutated TGF-β1 allele (TGF-β1−/− donor cells) and with actin primers to ascertain the presence of material.